银杏苦内酯B对创伤性脑损伤大鼠脑组织病理形态学的影响  

Effect of ginkgolide B on histopathology of brain tissue after traumatic brain injury in rats

作　　者：韩瑞璋 苏国军 于烽 赵保 盛文国 李斌 叶晶亮 马强 张建忠 

机构地区：[1]解放军98医院神经外科,湖州313000

出　　处：《第二军医大学学报》2013年第11期1262-1266,共5页Academic Journal of Second Military Medical University

摘　　要：目的探讨银杏苦内酯B(BN52021)对创伤性脑损伤大鼠脑组织病理形态学的影响。方法 48只SD大鼠随机分为4组:假手术组、模型组、低剂量BN52021组和高剂量BN52021组,每组12只。后3组制作液压打击脑损伤模型。低剂量BN52021组和高剂量BN52021组于模型制作当天开始分别腹腔注射1mg/kg和5mg/kg BN52021,每天1次,连续7d。第7天治疗后取脑组织进行形态学及免疫组织化学检测和超微结构观察。结果与假手术组相比,模型组大鼠脑组织神经元相对数量减少(P<0.05),OX-42阳性小胶质细胞和星形胶质细胞数量增加(P<0.05),caspase-3阳性细胞数增多(P<0.05);电镜下可见神经元染色质边集,部分出现核碎裂、核溶解,线粒体变圆变大,空泡形成,嵴消失,内质网增宽,溶酶体增多,核膜皱褶。低剂量和高剂量BN52021组与模型组比较,小胶质细胞和星形胶质细胞比例减少(P<0.05),caspase-3阳性细胞数减少(P<0.05),超微结构明显改善;高剂量组较低剂量组改善更加明显。结论 BN5202对创伤性脑损伤大鼠脑组织具有保护作用。Objective To investigate the neuroprotective effect of ginkgolide B （BN52021） on the histopathology of brain tissue after traumatic brain injury in rats. Methods Forty-eight healthy SD rats, weighing 250 g, were evenly randomized into 4 groups： sham control group, model group, low dose BN52021 group and high dose BN52021 group. Rats in the latter 3 groups were made into fluid percussion brain injury models. After operation, the rats in the low and high dose BN52021 groups were treated with BN52021 （low dose： 1 mg/kg, ip, high dose： 5 mg/kg, ip, once daily for 7 days）. On the 7th day after treatment, cerebral tissues were harvested from each group, and the histopathological changes of brain tissue were observed by Fast blue, electron microscope and immunohistochemical method. Results Compared with sham control group, model group had significantly decreased neurons （P〈0.05）, increased OX 42 immunoreactive microglial cells and astrocytes （P〈0. 05）, and cells positive for caspase-3 （P〈0.05）. Electron microscope found chromatin aggregation, nuclear fragmentation, rounder and larger mitoehondria, void formation and disappeared cristae of mitochondria, endoplasmic reticulum hypertelorism, increased lysosomes, and nuclear membrane folding. Compared with model group, the low and high dose BN52021 groups had significantly decreased proportions of microglial cells and astrocytes （P〈0.05）, significantly decreased caspase-3 positive cells （P〈0.05）, and improved ultrastructure, with the improvement in the high dose group being more notable than that in the low dose group. Conclusion BN52021 has protective effect on the morphology of brain tissue in rats with traumatic brain iniurv.

关 键 词：银杏苦内酯B 脑损伤 病理学 免疫组织化学 

分 类 号：R651.15[医药卫生—外科学]