共聚焦显微镜下真菌菌丝鉴别分析  

Identify the fungal hyphae by confocal microscope

作　　者：黎明[1] 姚晓明[1] 曹端荣[1] 聂丹瑶[1] 林宝涛[1] 

机构地区：[1]暨南大学附属深圳市眼科医院,深圳518000

出　　处：《中国实用眼科杂志》2013年第11期1420-1424,共5页Chinese Journal of Practical Ophthalmology

摘　　要：目的采用角膜激光共聚焦显微镜对真菌性角膜溃疡患者进行观察，对共聚焦显微镜下的真菌菌丝与其他组织结构进行鉴别。方法对122例（122只眼）真菌性角膜溃疡患者进行角膜激光共聚焦显微镜检查，对其共聚焦显微镜图像进行研究总结，对类似结构进行对比研究及鉴别。结果共聚焦显微镜下真菌菌丝需与角膜内的六类结构（神经纤维、角膜前弹力层崩解后形成的网状结构、新生血管、角膜缝线、树突状细胞、药物结晶结构）进行鉴别，角膜类的六类结构各有其特征。结论在熟悉角膜各层正常结构的情况下，通过共聚焦显微镜动态观察真菌菌丝与周围组织结构的关系，理解真菌菌丝与周围组织细胞的关系，将真菌茵丝与角膜内的六种结构进行鉴别，并不困难。Objective To observe the fimgal corneal ulcer by corneal confocal microscopy and identify the fungal hyphae and other organizational structure under confocal microscope. Methods One hundred and twenty-two cases （122 eyes） of fungal corneal ulcer in patients were examined by laser confocal microscopy. The characters of fungal hyphae images on the confocal microscopy were summarizes, and identified with the similar structure. Results The fungal hyphae were identified with the six intracorneal structures （nerve fibers, the mesh structure of Bowman＇s layer disintegrat- ing, neovascular, corneal suture, dendritic cells, drug crystal structure） by the confocal microscopy and were identified. There were characteristics of six intracorneal structures respectively. Conclu- sions It is not difficult to identifying the fungal hyphae with the six intracorneal structures in the fa- miliar with corneal layers of normal structure and observation on the relationship between fungal hy- phae and surrounding tissue structure by confocal microscopy and dynamic.

关 键 词：真菌菌丝 共聚焦显微镜 鉴别 真菌性角膜溃疡 

分 类 号：R285.5[医药卫生—中药学]