盐度对银鲳血清渗透压、过氧化氢酶及鳃离子调节酶活力的影响  

Effects of salinity on serum osmolality,catalase and gill ion-regulatory enzyme activities in silver pomfret

作　　者：施兆鸿[1,2] 张晨捷[1,2] 彭士明[1] 王建钢[1] 高权新[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部东海与远洋渔业资源开发利用重点实验室,上海200090 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《水产学报》2013年第11期1697-1705,共9页Journal of Fisheries of China

基　　金：国家科技支撑计划(2011BAD13B01);中央级公益性科研院所基本科研业务费项目(东2011M09号)

摘　　要：将银鲳幼鱼置于盐度14、25、36的海水中96h，检测不同盐度下银鲳幼鱼血清渗透压、过氧化氢酶（CAT）以及鳃泡膜质子泵（VHA）、Ca^2＋-ATP酶、碳酸酐酶（CA）活力随着时间推移而变化的情况。结果显示，血清渗透压变化和盐度呈正相关，并出现波动变化，变化范围为271～379mOsm／kg，96h时各盐度组血清渗透压均有恢复正常的趋势。高盐度（36）和低盐度（14）组CAT活力都显著升高而后逐渐恢复（P〈0．05），盐度36组8h时为各值中的最高点。鳃VHA活力变化：盐度14组先上升后回落，对照组基本保持平稳，盐度36组先下降而后恢复。盐度改变后，Ca^2＋-ATP酶活力都出现显著增强（P〈0．05），盐度14组比盐度36组的变化趋势更明显。盐度14和36组CA活力都出现显著增强（P〈0．05），其中盐度36组24h时为最高值。分析实验数据发现，血清渗透压与鳃VHA活力呈极显著负相关，与CA活力在低盐度适应时呈极显著负相关（P〈0．01），而盐度14组血清渗透压与Ca^2＋-ATP酶的变化呈基本相反趋势。结果表明，银鲳幼鱼在适应不同盐度时，血清渗透压会伴随盐度升降而改变，并激活CAT，而鳃VHA、Ca^2＋-ATP酶、CA在渗透压调节过程中有重要作用。The juvenile silver pomfret were cultured in water with salinity 14,25 and 36,respectively, for 96 h. And their serum osmolality, catalase （ CAT ） and gill V-H^＋-ATPase （ VHA ）, Ca^2＋-ATPase, carbonic anhydrase（CA） activities were tested in different safinity over time in this experiment. In this study, the serum osmolality which fluctuated was positively correlated to salinity, and its variation range is 271 -379 mOsm/kg. The serum osmolality of different salinity would return to normal at 96 h. CAT activities of salinity 36 and 14 returned to normal after they rose significantly（P 〈 0.05） ;the highest point of them was salinity 36 at 8 h. VHA activities rose,then recovered in salinity 14 ,kept stable in control salinity,fell down then recovered in salinity 36. Ca^2＋-ATPase activities increased significantly when salinity changed （P 〈 0.05） ,and the variation trend in salinity 14 was more identifiable than that in salinity 36. CA activities in salinity 14 and 36 both increased significantly（P 〈0.05）, and the highest point was found in salinity 36 at 24 h. Through analysis of the data, the serum osmolality was significantly negatively correlated to gill VHA activity （ P 〈 0.01 ）, and it was significantly negatively correlated to CA activity （ P 〈 0.01 ） in low salinity adaptation（ 14 and 25 ）. It also illustrated a contrary trend between serum osmolality and Ca^2＋-ATPase activity in salinity 14. This research proved that serum osmolality which changes with salinity, will activate CAT when silver pomfret adapts to different salinity. And VHA, Ca^2＋ -ATPase and CA are playing important roles in osmoregulation.

关 键 词：银鲳 盐度 渗透压 ATP酶 碳酸酐酶 

分 类 号：Q591.9[生物学—生物化学] S965[农业科学—水产养殖]