赖氨酸对团头鲂幼鱼生长、血清生化及游离必需氨基酸的影响  

Effects of lysine on growth,physiological and biochemical indexes of blood and essential amino acids of serum in juvenile blunt snout bream(Megalobrama amblycephala)

作　　者：廖英杰[1,2] 刘波[1,2] 任鸣春[2] 崔红红[1,2] 谢骏[1,2] 戈贤平[1,2] 

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,江苏无锡214081

出　　处：《水产学报》2013年第11期1716-1724,共9页Journal of Fisheries of China

基　　金：公益性行业(农业)科研专项(201003020);现代农业产业技术体系建设专项(CARS-46)

摘　　要：为探讨饲料中不同水平赖氨酸对团头鲂幼鱼生长、血清生化及血清中游离必需氨基酸的影响,选用初始均重(3.34±0.03)g的团头鲂幼鱼540尾,随机分成6个实验组,每个实验组3个重复,每个重复30尾鱼,分别投喂赖氨酸含量为1.29%、1.71%、2.09%、2.48%、2.88%、3.27%的饲料,养殖实验为10周。研究表明:(1)与对照组相比,2.48%赖氨酸实验组显著提高了团头鲂幼鱼的鱼体末重、增重率、特定增重率、氮沉积率、蛋白质效率和蛋白沉积率,显著降低了饵料系数,但不影响存活率;(2)2.09%赖氨酸实验组显著提高鱼体蛋白含量和降低脏体比系数,2.88%、3.27%赖氨酸实验组显著降低了粗灰分含量;(3)3.27%赖氨酸实验组显著提高了血清中谷丙转氨酶和总蛋白含量;2.14%赖氨酸实验组显著降低了血清中白蛋白的含量;2.88%赖氨酸实验组显著降低了血清中尿素含量;(4)除血清亮氨酸和缬氨酸之外,2.88%赖氨酸实验组显著提高了血清中必需氨基酸含量;同时,随着饲料中赖氨酸水平的增加,血清中总必需氨基酸呈先升高后趋于平缓的趋势。以鱼体特定增重率和蛋白沉积率为评价指标,经折线模型回归分析得到团头鲂幼鱼饲料中赖氨酸的适宜添加量分别为饲料干重的2.36%(饲料粗蛋白含量的6.96%)和2.22%(饲料粗蛋白含量的6.53%)。A 10-week feeding trial was conducted to investigate effects of lysine on growth,physiological and biochemical indexes of blood and essential amino acids of plasma in juvenile Megalobrama amblycephala. Six isonitrogenous and isoenergetic semi-purified diets were formulated to contain graded dietary lysine levels, and the values were 1. 29% , 1. 71% , 2. 09% , 2. 48% , 2. 88% and 3. 27% , respectively. At the initiation of experiment, fish[ （ 3.34 ± 0.03 ） g ]were randomly chosen and sorted into eighteen cages with 30 fish per cage. Each experimental diet was randomly assigned to triplicate cages in a completely randomized design. At the end of feeding trial, final body weight （ FBW ）, weight gain rate （ WRG）, specific growth rate （ SGR）, Nitrogen retention efficiency （ NRE ）, protein productive value （ PPV ） and protein efficiency ratio（PER）of fish fed diet containing 2.48% lysine were significantly higher than those fish fed 1.29% lysine diet while feed conversion ratio（FCR）showed an adverse tendency,but no significant difference was observed in survival rate （SR）of fish fed different dietary methionine levels. Increased crude protein and reduced viscerosomatic ratio（VR） were observed in fish fed 2.48% lysine diet. Ash of whole body of fish fed 2.88% and 3.27% lysine diet was higher than those fish fed 1.29% lysine diet. Alanine aminotransferase （ALT）and total protein （TP）contents were significantly improved with increasing lysine levels up to 3.27%. Urea content in plasma of fish fed 2.94% lysine diet was lower than those fish fed 1.29% lysine diet. Fish fed the 2.88% lysine diet demonstrated a significant improvement in plasma lysine content and total essential amino acid except for Leu and Val. On the basis of SGR and PPV, the optimum dietary lysine requirement of juvenile M. amblycephala was estimated to be 2.36% of diet （6.94% of protein） and 2. 22% of diet （6. 53% of protein）, respectively, using broken-line regression analysis.

关 键 词：团头鲂 赖氨酸 需要量 生长 

分 类 号：Q591.9[生物学—生物化学] S965[农业科学—水产养殖]