血红蛋白分析的方法学评价  

Evaluation of major methods of hemoglobin analysis

作　　者：夏勇[1] 熊礼宽[1] 

机构地区：[1]深圳市宝安区妇幼保健院中心实验室,518133

出　　处：《中华检验医学杂志》2013年第10期950-953,共4页Chinese Journal of Laboratory Medicine

基　　金：广东省深圳市科技计划项目（201003340）

摘　　要：血红蛋白病是由于正常血红蛋白比例失衡或异常血红蛋白出现所引起的一种遗传性血液病.血红蛋白分析对该病的筛查和诊断有着重要意义.从20世纪初开始,新的血红蛋白分析技术日益广泛应用于临床,包括琼脂糖凝胶电泳、高效液相色谱法和毛细管电泳法,质谱法也逐渐开始被应用.这些方法都有各自的特点,合理的联合使用能够使得血红蛋白分析更加科学可靠.Hemoglobinopathy is a kind of hereditary hematonosis caused by the disproportion among normal hemoglobins or formation of abnormal hemoglobins. Hemoglobin analysis plays an important role in screening and diagnosing hemoglobinopathy. Emerging methods have been applied continuously to hemoglobinometry since the early 20th century. Nowadays, agarose gel electrophoresis, high performance liquid chromatography and capillary electrophoresis are widely used with mass spectrometry as the latest applied method. All these methods have their own features . Appropriate combination of these methods will lead to more scientific and reliable resuhs.

关 键 词：血红蛋白测定 电泳 琼脂凝胶 色谱法 高压液相 电泳 毛细管 质谱分析法 

分 类 号：R446.1[医药卫生—诊断学]