α2A-肾上腺素能受体基因-1296位点多态性与前庭功能敏感的相关性研究  

The correlation research between the polymorphism of genotype of site-1296 in α2A-AR receptor gene and the susceptibility of vestibular function

作　　者：郭睿[1] 李佳[1] 秦贺[1] 蔡庆[1] 王骞翊 龚维熙[1] 

机构地区：[1]中国人民解放军空军总医院耳鼻咽喉科,北京100142

出　　处：《临床耳鼻咽喉头颈外科杂志》2013年第22期1269-1271,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：总后十二五重大课题项目(No:AKJ11J003)

摘　　要：目的:探讨α2A-肾上腺素能受体(α2A-AR)基因-1296位点基因型与前庭功能敏感相关性。方法:取飞行学员中44例前庭反应敏感和50例前庭功能反应不敏感者分为两组作为研究对象,对两组成员分别进行DNA提取及扩增并进行基因序列测定。采用SPSS17.0统计软件。基因频率采用基因计算法处理,研究对象与Hardy-Weinberg平衡的符合程度及两组间不同基因型比较、等位基因频率比较采用χ2检验。结果:在α2A-AR基因调控区第-1296位点存在G/C多态性,经测序获得了GG、GC和CC 3种基因型,其中GG基因型在前庭反应敏感组中的表达远远大于前庭反应不敏感组,经统计学处理两组间基因型构成比及两组间等位基因频率差异有统计学意义(P<0.05)。结论:α2A-AR受体基因-1296位点多态性与前庭功能敏感性可能相关。Objective：To investigate the correlation between the polymorphism of genotype of site-1296 in α2A-AR receptor gene and the susceptibility of vestibular function. Method： Ninety-four blood samples were col- lected from pilot cadets, consisting of susceptible and tolerance groups to vestibular function. Genomic DNA was isolated, and the coding region of α2A-AR receptor gene was amplified by polymerase chain reaction（PCR）. The PCR products were analyzed by gene sequencing. Gene frequency was calculated, and, the coincidence between the polymorphism of α2A-AR receptor gene in the groups and Hardy-Weinberg balance was evaluated, The allele fre- quency of the two groups was compared by Chi square test. Resuit：G/C polymorphism was existed in Site-1296 of α2A-AR gene regulation zone, including GG.GC,CC. The express of GG Genotype in susceptible group exceeded that of the other group. There were significance differences in both genotype constituent ratio and alleles frequency of the two groups. Conclusion：The polymorphism of genotype of site--1296 in α2A-AR receptor gene is possibly correlated with the susceptibility to vestibular function.

关 键 词：α2A-肾上腺素能受体基因 基因型 前庭功能 

分 类 号：R339[医药卫生—人体生理学]