单抗英夫利西对克罗恩病人T细胞亚群及相关细胞因子的影响  

Alterations of T cell subsets and cytokines levels in patients with Crohn's disease following Infliximab therapy

作　　者：王红[1] 李呈贞[1] 钮晓音[1] 王正廷[2] 范嵘[2] 席晔斌[1] 赵晴[1] 程文婧[1] 钟捷[2] 陈广洁[1] 

机构地区：[1]上海市免疫学研究所,上海交通大学医学院免疫学教研室 [2]上海交通大学医学院附属瑞金医院,上海200025

出　　处：《现代免疫学》2013年第6期456-460,共5页Current Immunology

基　　金："985"三期项目基础临床交叉团队项目;上海市科委基础重点项目(11JC1411602);上海市教委科研创新重点(12ZZ103;J50207);上海市卫生局基金(2011177)

摘　　要：观察以英夫利西单抗(IFX)治疗克罗恩病(CD)前后,患者外周血Th1、Th17、MΦ细胞、Treg细胞及相关细胞因子、转录因子表达水平的变化,探讨在IFX治疗CD中的免疫调节作用。采用FACS、RT-PCR、ELISA测定外周血T细胞亚群细胞数量、相关细胞因子和转录因子的表达水平。结果显示,治疗后CD患者外周血中Th1、Th17细胞亚群的比例均下降,Treg细胞亚群的比例上升;血浆细胞因子IFN-γ、IL-17、骨桥蛋白(OPN)的水平下降;相应的转录因子T-bet和RORC有明显下降,而Foxp3有显著增加。研究表明IFX可通过免疫调节和抗炎症作用治疗CD。In order to study the effect of Infliximab（IFX） on patients with Crohn＇s disease （CD）, we used flow eytometry （FACS）, real-time Polymerase Chain Reaction （RT PCR） and enzyme linked immunosorbent assay （ELISA） to analyze per centages of different T cell subsets in the peripheral blood, levels of relevant cytokines and transcription factors before and after IFX treatment. The results showed that after IFX treatment, the levels of IFN-γ, IL 17 and OPN in the plasma decreased, meanwhile, the percentage of Thl and Th17 subsets in the peripheral blood also declined, On the contrary, the Treg subset as- cended, and correspondingly, the transcription factor T-bet and RORC were downregulated while Foxp3 was upregulated. All the results indicate that IFX can treat CD through immunoregulation and antiqnflammation.

关 键 词：英夫利西单抗 克罗恩病 T细胞亚群 细胞因子 

分 类 号：R392.12[医药卫生—免疫学]