染矽尘大鼠支气管肺泡灌洗液中细胞因子的变化  

Changes of cell factor in bronchoalveolar lavage fluid in rats exposed to silica

作　　者：张玮[1] 王瑞[1] 王欣 周效宝[3] 王辉[1] 张海东[1] 刘正亮[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250062 [2]山东地方病防治研究所 [3]山东省安康医院

出　　处：《中华劳动卫生职业病杂志》2013年第11期801-805,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目（30872094）;山东省科技攻关项目（2013GSF11828）

摘　　要：目的探讨染矽尘大鼠早期支气管肺泡灌洗液（BALF）中炎性细胞因子的变化。方法将实验大鼠随机分为对照组、15、30、60mg／ml染矽尘实验组，每组42只。一次性气管内注入染尘，不同处理后1、3、7、14、21、28d每组分别处死7只大鼠，收集BALF，采用ELISA法测定肿瘤坏死因子（tumornecrosisfactor，TNF）、白细胞介素（interleukin，IL）．1、IL-6、IL-16、巨噬细胞炎性蛋白-1（macrophage in flammatory protein-1，MIP-I）、单核细胞趋化因子-1（monocyte chemoattractant protein．1，MCP-1）、肿瘤坏死因子（tumornecrosis factor，TNF．d）、转化生长因子（transforming growth factor-β，TGF．B）的浓度。结果各剂量组BALF中炎性细胞因子在各时间点较对照组均有不同程度的增高，在染尘初期（1-3d时）BALF中IL-1随染尘剂量的增加而增高的趋势较明显，14d时IL-6、IL-16随染尘剂量增加而增高的趋势较明显；染尘组IL-1、IL-6、IL-16均在14d时出现浓度峰值。不同剂量染尘组MIP-1α、MCP-1浓度的差异有统计学意义（RMP-1α=30．106，P〈0．01；FMCP-1=17．193，P〈0．01）。各组不同染尘时间MCP-1浓度的差异无统计学意义（F=0．618，P〉0．05）；在1-14d BALF中TNF-α随接尘剂量的增加而增高，剂量反应关系明显，差异有统计学意义（P〈0．05o各剂量组TNF-α在 14d出现高峰。高剂量组14、21、28dBALF中TGF-β水平均高于低剂量组，14、28dBALF中TGF-β水平均明显高于中剂量组，差异均有统计学意义（P〈0．05）。结论IL-1、IL-6、IL-16、MIP-1α、MCP-1、TNF-α在矽肺炎症发生发展过程中发挥了作用，TGF-β可能与纤维化过程有关联。Objective To investigate the changes in the levels of inflammatory cytokines in bronchoalveolar lavage fluid （BALF） in rats exposed to silica dust. Methods Experimental rats were randomly divided into control group and three experimental groups （doses of dust： 15, 30, and 60 mg/ml）, with 42 rats in each group. Each rat in the control group was treated with 1 ml of normal saline by intratracheal instillation, while each rat in the experimental groups was exposed to 1 ml of silica suspension by a single intratracheal instillation. Seven rats in each group were killed at 1, 3, 7, 14, 21, and 28 days after exposure, and then BALF was collected. Enzyme-linked immunosorbent assay was used to measure the levels of interleukin （IL）-I, IL-6, IL-16, macrophage inflammatory protein-1 alpha （MIP-1α）, monocyte chemoattractant protein-! （MCP-1）, tumor necrosis factor-or （TNF-α）, and transforming growth factor-β （TGF-β）. Results The levels of cytokines in each experimental group were higher than those in the control group at any time point. In the early eage of exposure （day 1-3）, BALF IL-1 level increased significantly with the increase in dust dose, and on day 14, BALF IL-6 and IL-16 levels increased significantly with the increase in dust dose; the levels of IL-1, IL-6, and IL-16 in the experimental groups reached the peak on day ld. There were significant differences in the levels of MIP-lct and MCP-1 between the experimental groups （FMIP-la=30.106, P〈0.01; FMCP-1=17.193, P〈0.01）. In each group, the level of MCP-1 varied significantly at different time points （F=0.618, P〉0.05）. On day 1-14, BALF TNF-α level increased with the increase in dust dose, with a significant dose-response relationship （P〈 0.05）. In each experimental group, TNF-a level reached the peak on day 14. On days 14, 21, and 28, the high- dose group had significantly higher BALF TGF-β levels than the low-dose group （P〈0.05）; on days 14 and 28,the high-dose group had significantly higher BALF T

关 键 词：二氧化硅 大鼠 支气管肺泡灌液 白细胞介素 转化生长因子 

分 类 号：R135.2[医药卫生—劳动卫生]