表皮生长因子基因多态性与矽肺易感性  

The relationship between polymorphisms of epidermal growth factor gene and silicosis

作　　者：江冬萍[1] 王朝阳[2] 范雪云[1] 

机构地区：[1]河北联合大学公共卫生学院,唐山063000 [2]唐山钢铁集团有限责任公司医院

出　　处：《中华劳动卫生职业病杂志》2013年第11期820-824,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：河北省科技厅资助项目（09276195D）

摘　　要：目的探讨表皮生长因子（epidermal growthfactor，EGF）基因3个位点（G-61A、D784V、R431K）的基因多态性与矽肺易感性的关系。方法采用病例对照研究方法，选择已确诊的116例Ⅰ期矽肺患者为病例组，以同一性别、民族、接触相同性质粉尘，年龄、累积接尘工龄相近的非矽肺接尘工人149例为对照。采集外周静脉血，盐析法提取DNA，应用聚合酶链一限制性片段长度多态性（PCR-RFLP）技术检测EGF3个多态位点的基因型和等位基因频率，分析其在病例组及对照组的分布。结果病例组G．61A位点GG、GA和AA基因型频率分别为50．9％、34．5％、14．7％，与对照组（35．6％、44．3％、20．1％）比较，差异有统计学意义（X^2=6．283，P=0．048）；病例组和对照组携带的A等位基因的分布频率分别为31．9％和42．3％，差异有统计学意义（X^2=5．554，P=0．018）。携带G．61A位点G等位基因患病风险增加1．564倍（0R=1．564，95％CI：1．092。2．024）。病例组D784V位点AA、AT和TT基因型频率分别为58．6％、34．5％和6．9％，与对照组（65．1％、31．5％、3．4％）比较，差异无统计学意义（X^2=2．278，P=0．320）。病例组R431K位点GG、GA和AA基因型频率分别为56．9％、39．7％、3．4％，与对照组（55．0％、39．6％、5．4％）比较，差异无统计学意义（X^2=0．572，P=0．751）。结论EGF基因G．61A位点的基因多态性与矽肺易感性存在关联，携带GG基因型的接尘工人患矽肺的危险性相对增加。未发现EGFD784V、R431K位点的基因多态性与矽肺的发病有关。Objective To investigate the relationship between epidermal growth factor （EGF） gene polymorphisms at G-61A, R431K, and D784V and susceptibility to silicosis. Methods In a case-control study, 116 patients diagnosed with stage I silicosis were included in the case group, and 149 workers without silicosis of the same gender and nationality, exposed to the same nature of dust, and with similar age and cumulative time of dust exposure were included in the control group. Peripheral venous blood was collected, DNA was extracted by salting out, polymerase chain reaction-restriction fragment length polymorphism was used to identify the genotypes at three polymorphic loci of EGF and the allele frequencies, and their distributions in the case group and control group were analyzed. Results The genotype frequencies of G-61A GG, GA, and AA in the case group were 50.9%, 34.5%, and 14.7%, respectively, and significant differences were found when comparing the data with those in the control group （35.6%, 44.3%, and 20.1%）, （X^2=6.283, P=0.048）. The distribution frequencies of allele A in the case group and control group were 31.9% and 42.3%, respectively, and the difference between the two groups was statistically significant （X^2=5.554, P=0.018）. The risk of silicosis in workers carrying allele G at G-61A was increased by 1.564 times （OR=1.564, 95%CI： 1.092-2.024）. The genotype frequencies of D784V AA, AT, and ＇IT in the ease group were 58.6%, 34.5%, and 6.9%, respectively, versus 65.1%, 31.5%, and 3.4% in the control group, and the differences between the two groups were not statistically significant （X^2=2.278, P= 0.320）. The genotype frequencies of R431K GG, GA, and AA in the case group were 56.9%, 39.7%, and 3.4%, respectively, versus 55.0%, 39.6%, and 5.4% in the control group, and the differences between the two groups were not statistically significant （X2=0.572, P=0.751 ）. Conclusion The EGF gene polymorphism at G-61A is associated with susceptibility to silicosis, and the risk of silicos

关 键 词：矽肺 表皮生长因子 多态性 单核苷酸 疾病易感性 

分 类 号：R135.2[医药卫生—劳动卫生]