白细胞介素-10基因修饰对骨髓间充质干细胞体外免疫效应的影响  

Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells

作　　者：方针强[1] 戴玮 贾维胜[1] 何凡[1] 沈崇星[1] 刘永亮[1] 周锋[1] 叶钢[1] 

机构地区：[1]第三军医大学新桥医院泌尿外科,重庆400037 [2]重庆石油医院内科,重庆400037

出　　处：《重庆医科大学学报》2013年第11期1300-1302,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81200500);重庆市科委自然科学基金计划资助项目(编号:CSTC;2009BB5154)

摘　　要：目的:研究白细胞介素-10(interleukin-10,IL-10)基因修饰的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)体外免疫效应。方法:RT-PCR克隆大鼠IL-10基因并构建逆转录病毒pLNCX-IL-10,转染大鼠BMMSCs;流式细胞仪检测IL-10基因修饰的BMMSCs(BMMSCs IL-10)体外对T淋巴细胞亚群的影响,CCK-8法检测BMMSCs IL-10及其培养上清液对体外混合淋巴细胞增殖的影响。结果:BMMSCsIL-10能明显减少CD4+T,降低CD4+/CD8+比值,比BMMSCs作用明显(P=0.000);BMMSCsIL-10及其培养上清液比BMMSCs更能明显抑制T淋巴细胞增殖反应(P=0.000)。结论:IL-10基因修饰能增强BMMSCs的体外免疫抑制作用。Objective：To study the in vitro immunosuppression of interleukin-10（IL-10） modified bone marrow mesenchymal stem cells （BMMSCsIL-10）. Methods：IL-lO was cloned by RT-PCR and was used to construct pLNCX-IL-10. The constructed retrovirus was transfected into rat BMMSCs. T lymphocyte subsets in vitro influenced by BMMSCsIL_m0 were detected by flow cytometry. Effects of BMMSCsIL-10 and its culture supernatant on in vitro mixed heterologous T lymphocyte proliferation were detected by CCK-8. Results： BMMSCsnIL-10 significantly reduced CD4＋T and lowered the ratio of CD4＋/CD8＋, more intense than BMMSCs（P=0.000）. BMMSCsIL-10and its culture supernatant significantly inhibited T lymphocyte proliferation,more intense than BMMSCs（P=0.000）. Conclusions： IL-IO modification can enhance the in vitro immunosuppressive effect of BMMSCs.

关 键 词：骨髓间充质干细胞 白细胞介素-10 免疫抑制 

分 类 号：R392.4[医药卫生—免疫学]