蛋白质组学方法分析多T迷宫训练后的小鼠空间记忆蛋白  

Spatial Memory Proteins were Analyzed by MTM Training and Proteomics in Mice

作　　者：郑君芳[1] 陈慧敏[1] 宋然[1] 胡家[2] 沙莎[1] 贺俊崎[1] 

机构地区：[1]首都医科大学生物化学与分子生物学系,北京100069 [2]首都医科大学蛋白质研究平台,北京100069

出　　处：《中国生物化学与分子生物学报》2013年第11期1054-1060,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30900247);北京市属高等学校高层次人才引进与培养计划项目(No.CIT&TCD 2013 04187);北京市教委面上项目(No.KM201110025002);2010年度北京市优秀人才培养资助(No.2010D005018 000012);教育部留学回国人员科研启动基金资助项目(第42批)基金资助~~

摘　　要：应用双向凝胶电泳结合质谱鉴定和数据库检索,分析比较C57BL/6J小鼠在多T迷宫(MTM)训练和记忆测试组与未训练组海马蛋白表达的差异,探讨与MTM空间记忆相关的蛋白质.C57BL/6J小鼠经MTM训练后,可对相应的空间线索保持记忆能力,其海马蛋白质表达存在明显差异,14个蛋白质与MTM空间记忆形成显著相关.其中,6个蛋白点表达显著上调,8个蛋白点表达水平显著降低.这些蛋白按功能可分为6类:细胞骨架相关蛋白,物质运输相关蛋白,蛋白合成相关蛋白,能量和物质代谢相关蛋白,信号转导相关蛋白,通道蛋白.这些空间记忆形成相关蛋白的研究深化了对空间记忆机制的认识,为研究和治疗认知相关疾病提供了新靶标.To investigate the proteins related with spatial memory, hippocampal proteins from C57BL/6J mice after multiple T maze （MTM） training task and probe trial were extracted and subjected to 2D gel electrophoresis. Protein quantification was analyzed and the differentially expressed spots were identified using mass spectrometry and MASCOT database search. Hippoeampal proteins from C57BL/6J mice after MTM probe trial were significantly different. 14 proteins were found to be closely related with spatial memory formation. Expressions of 6 proteins in hippocampus of C57BL/6J mice increased significantly; expressions of 8 proteins in hippocampus of C57BL/6J mice decreased significantly. These identified proteins could be divided into six categories according to their functions： cytoskeleton, transporter, protein synthesis, energy metabolism, signal transduetion and channel protein. These proteins deepened insights into the spatial memory mechanism and provided new targets for the study and treatment of cognitive diseases.

关 键 词：空间记忆 海马蛋白 蛋白质组学 质谱法 

分 类 号：Q51[生物学—生物化学]