气相色谱法测定氟康唑原位胶化滴眼液滴眼后兔泪液和房水中的药物浓度(英文)  

Determination of fluconazole in tears and aqueous humors after topically applied to rabbit eyes by gas-liquid chromatography

作　　者：张俊杰[1] 谢雷克[1] 陈祖基[1] 徐岩[1] 

机构地区：[1]河南省眼科研究所,郑州450003

出　　处：《眼科研究》2000年第5期385-388,共4页Chinese Ophthalmic Research

摘　　要：目的 建立一种快速、灵敏和可靠的气相色谱法以评价氟康唑原位胶化眼部给药系统兔眼应用后氟康唑的生物利用度。方法 应用大口径毛细管柱。检测为氮磷检测器。将泪液处理后和房水用甲醇溶解注入色谱仪中。采用外标法定量。结果 每个样品的分析时间小于10min,最小可检测浓度为0.01μg/ml,在0.1至20.0μg/ml范围内线性关系良好。药物从泪液和房水中的回收率分别为99.0%和94.8%。结论 此方法可用于测定兔眼泪液和房水中的氟康唑浓度,对于评价氟康唑局部应用后眼部生物利用度是一个可行的、快速的、有效可靠的方法。Objective To develop a rapid,sensitive and reliable high perf ormance gas chromatography to evaluate the fluconazole (FCZ) bioavailability after topically applied FCZ in situ gelling ophthalmic delivery system to the rabbiteyes .Methods A megabore capillary column(30m long and inner diameter of 0.530 mm) with a 0.88μm thick bonded liquid phase was used.Detector was nitrogen selective type. Tears adsorbed to the filter paper was directly by methanol with no evaporation stage prior to analysis .Aqueous humors was extracted by ethylacetate. Results The duration of each analysis was less 10 min and the minimum detectable concentration was 0.01 μg/ml. The assay was linear from 0.1 to 20 μg/ml. The average recoveries from tears and aqueous humors were 99.0% and 94.8%,respectively.Conclusion This method can be used to determine rabbit tears and aqueous humors levels of fluconazole and was practicable approach for evaluating intraocular pharmacokinetics after topically applied to rabbit eyes.

关 键 词：氟康唑原位胶化滴眼液 药物浓度 气相色谱法 

分 类 号：R965[医药卫生—药理学]