电极介导的菌紫质干膜微分光电流响应的整流性质(英文)  

Electrode-mediated Rectification of The Differential Photocurrent Response of Dry Bacteriorhodopsin Film

作　　者：徐兵[1] 韩金多[1] 王敖金[2] 胡坤生[2] 

机构地区：[1]上饶师范学院生命科学系,上饶334001 [2]中国科学院生物物理研究所,北京100101

出　　处：《生物化学与生物物理进展》2013年第11期1160-1164,共5页Progress In Biochemistry and Biophysics

摘　　要：在长方形光脉冲光照下,菌紫质(bacteriorhodopsin,BR)干膜组装成夹层光电池具有微分光电流响应.在氧化铟锡(ITO)导电玻璃/BR膜/封口膜/不锈钢形成的干膜电池下可观察到整流特性,而在不锈钢/BR膜/封口膜/ITO导电玻璃形成的干膜电池下则观察不到整流特性,这说明是电极介导的整流.平衡电压测定表明:工作电极/BR膜表面与对电极/BR膜表面有不同的性质,电极的界面效应控制了BR的取向.酸与碱产生的瞬间电流极性也证实了电极整流行为的存在.这些结果将有助于了解BR膜的微分光电响应.Dry bacteriorhodopsin （BR） film was fabricated into sandwich photocell and has differential photocurrent response to rectangular light pulse. And rectification was observed in the dry photocell of indium-tin oxide/BR film/Parafilm/stainless steel, but not of the steel/BR filrn/Parafilm/ ITO. This is an evidence of electrode-mediated rectification. Measurement of equilibrium potential suggests that the working electrode/BR film interface have different property from that of its counter electrode/Parafilm/BR film interface. The interracial effect of the electrodes may dominate over that of the orientation of BR. Polarities of acid or base induced transient current confirmed the presence of rectification behavior of the electrode. The results will help to understand the mechanism of differential response of BR film.

关 键 词：微分光电流 极性 菌紫质膜 电极介导整流 界面效应 

分 类 号：Q632[生物学—生物物理学]