腹腔注射链脲佐菌素构建糖尿病大鼠模型  

Diabetic rat model built by intraperitoneal injection of streptozotocin

作　　者：曾新生[1] 姚微微[1] 丁芝祥[1] 李政[1] 高翔[1] 孙建国[1] 彭燕一[1] 

机构地区：[1]桂林医学院附属医院眼科,广西桂林541001

出　　处：《华夏医学》2013年第5期895-897,共3页Acta Medicinae Sinica

基　　金：桂林市科学研究与技术开发计划项目(20080407)

摘　　要：目的:建立糖尿病大鼠模型以便研究糖尿病视网膜病变。方法:采用少量多次腹腔注射链脲佐菌素(STZ)制造大鼠糖尿病模型。设对照组,体重比20,30,40mg/kg STZ组,每组5只大鼠。1个月后,尿糖在以上,血糖高于16.7mmol/L为造模成功。结果:30,40mg/kg STZ组全部大鼠都造模成功;20mg/kg STZ组有2只大鼠血糖低于8.2mmol/L,尿糖+,造模不成功。结论:少量多次腹腔注射链脲佐菌素(STZ)是糖尿病大鼠模型构建的较好方法。Objective： To establish the model of diabetic rats for the study of diabetic retinopathy. Methods： Using a small dose, many times intraperitoneal injection of streptozotocin （STZ） to build rat diabetic model. Design control group, weight ratio of 20, 30 and 40 mg/kg STZ groups, 5 rats in each group. 1 month later, that urine glucose of rat was over Ⅲ, blood glucose was higher than 16.7 mmol/L was considered as successful diabetic rat model. Results： 30 and 40 mg/kg STZ groups, all the rats were successful diabetic model; 20 mg/kg STZ group, blood glucose of 2 rats below 8.2 mmol/L, urine glucose ＋, model failed. Conclusion： Small dose, many times intraperitoneal injection of streptozotocin （STZ） is a good method to construct the model of diabetic rats.

关 键 词：糖尿病 链脲佐菌素 大鼠 模型 

分 类 号：R587.2[医药卫生—内分泌]