非重型再生障碍性贫血患者Th17/Treg比例及相关细胞因子白细胞介素-6、-17和转化生长因子β1水平的临床意义  

Clinical significance of Th17/Treg in NSAA and the relationship with the levels of IL-6,IL-17 and TGF-β1

作　　者：胡令彦[1] 周永明[1] 陈英坤[1] 胡明辉[1] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院血液科,200437

出　　处：《临床内科杂志》2013年第9期636-638,共3页Journal of Clinical Internal Medicine

基　　金：国家自然科学基金资助项目（81001510）

摘　　要：目的 探讨非重型再生障碍性贫血（NSAA）患者外周血的Th17和调节性T（Treg）细胞比例异常变化及其与白细胞介素（IL）-6、IL-17、转化生长因子（TGF）-β1水平的相关性.方法 根据诊断标准收集NSAA患者78例,应用流式细胞术（FCM）检测其外周血Th17和Treg细胞比例,用酶联免疫吸附试验（ELISA）方法检测IL-6、IL-17和TGF-β1水平,选取20例健康者为对照组,并比较其差异及其相关性.结果 NSAA患者的Th17细胞比例高于对照组（P＜0.01）,Treg细胞比例低于对照组（P＜0.01）,Th17/Treg比例明显高于对照组（P＜0.01）.NSAA组IL-6水平明显高于对照组（P＜0.01）,NSAA组IL-17水平与对照组比较差异无统计学意义（P＞0.05）,NSAA组TGF-β1水平较对照组明显降低（P＜0.05）.Treg与Th17细胞无相关,Th17与IL-17水平有相关性.结论 Th17/Treg异常与非重型再生障碍性贫血发病有关.相关细胞因子TGF-β1、IL-6与Th17与Treg细胞比例的变化有关,可能是导致NSAA发生及发展的重要因素.Objective To explore the unbalanced Thl7/Treg in non severe aplastic anemia （NSAA） and the relationship with the levels of interleukin（IL）-6,IL-17 and Transforming growth factor （TGF）-β1.Methods A total of 78 NSAA patients and 20 healthy persons were inrolled in this study.The proportion of Treg cells（CD; CD25＋Foxp3 ＋） and Thl7 cells in peripheral CD4＋ T cells was analyzed by flow cytometry.The levels of plasma IL-6,IL-17 and TGF-β1 were measured by ELISA.The results were analyzed for the difference and relationship.Results The proportion of Th17 and Th17/Treg of NSAA group was higher than that in normal group（P 〈0.01）,and the proportion of Treg of CAA group was lower than that in normal group（P 〈 0.01）.The level of IL-6 in NSAA group was higher than that in normal group.The level of TGF-β1 in CAA group was lower than that in normal group.There was no difference of IL-17 in the two groups（P 〉0.05）.The proportion of Th17 showed the positive relationship with the level of IL-17 and the negative relationship with the proportion of Treg.Conclusion The unbalance of Th17/Treg in CAA patients maybe relate with the morbidity of NSAA.There is relationship in the TGF-β1,IL-6 and the proportion of Th17/Treg.the variety maybe one of the cause of NSAA.

关 键 词：非重型再生障碍性贫血 调节性T细胞 Th17细胞 转化生长因子-β1 白细胞介素-6 白细胞介素-17 

分 类 号：R752.120.5[医药卫生—皮肤病学与性病学]