基于HPLC-Q-TOF/MS的代谢组学方法用于解毒祛瘀滋阴方对系统性红斑狼疮干预作用的研究  

Study on intervention effect of Jieduquyuziyin prescription systemic lupus erythematosus by HPLC-Q-TOF /MS

作　　者：胡金波[1] 谷恒存[1] 丁志山[1] 姚立[1] 范永升[1] 丁兴红[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中国中药杂志》2013年第21期3747-3752,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81001515);高等学校博士学科点专项科研基金项目(20093322120001)

摘　　要：该文建立了基于高效液相色谱-四级杆-飞行时间质谱联用(HPLC-Q-TOF/MS)的代谢组学方法,研究系统性红斑狼疮(SLE)小鼠经解毒祛瘀滋阴方治疗后血清代谢物的变化,探讨SLE发病及药物作用机制。利用正交偏最小二乘法(orthogonal partial least squares,OPLS)对实验数据进行模式识别,发现对照组、SLE模型组、解毒祛瘀滋阴方治疗组和醋酸泼尼松治疗组的代谢图谱存在较明显的差异。根据OPLS载荷图,利用精确质量数结合二级质谱鉴定出traumatic acid,PAF,12(S)-HETE,15(S)-HETrE,Hepoxilin B3等12种差异代谢物。SLE小鼠经解毒祛瘀滋阴方治疗后,有效调节PAF,12(S)-HETE等差异代谢物的相对含量接近于对照组水平。代谢通路分析表明,SLE发病会导致体内不饱和脂肪酸和氨基酸代谢通路显著变化,而解毒祛瘀滋阴方对SLE紊乱的不饱和脂肪酸代谢通路具有一定修复调节作用。To establish a metabonomic method based on high performance liquid chromatography-quadrupole-time of flight mass spectrometry （HPLC-Q-TOF/MS）, in order to study the changes in serum metabolites of systemic lupus erythematosus （SLE） mice after treatment of Jieduquyuziyin prescription, the pathogenesis of SLE and mechanism of drug action. The orthogonal partial least squares （OPLS） was applied for the pattern recognition of experimental data, finding a significant difference in the control group, the SLE model group, the Jieduquyuziyin prescription-treated group and the prednisone acetate-treated group. According to the OPLS load diagram, 12 differential metabolites, including traumatic acid, PAF, 12 （S）-HEPE, 15（S）-HETrE and Hepoxilin B3 were identified by using accurate mass combined with MS/MS data. After treatment with Jieduquyuziyin prescription, the relative contents of PAF, 12（S）-HETE were close to the level of the control group. According to the analysis on metabolic pathway, SLE could cause significant changes in unsaturated fatty acid and amino acid metabolism pathway, while Jieduquyuziyin prescription has a effect in regulating disorder of unsaturated fatty acid metabolism pathway.keywords：metabolomics

关 键 词：代谢组学 系统性红斑狼疮 解毒祛瘀滋阴方 HPLC-Q-TOF MS OPLS 

分 类 号：R285.5[医药卫生—中药学]