芒果苷对慢性应激抑郁小鼠行为及海马脑源性神经营养因子表达的影响  

The effects of mangiterin on the improvement of behavior and expression of brain-derived neurotrophic factor in the hippocampus of chronic stress-induced depression model mice

作　　者：付燕燕[1] 宋远见[1] 杨宜华 段静雨 

机构地区：[1]徐州医学院基础学院生物学教研室,徐州221004 [2]药学院

出　　处：《中华行为医学与脑科学杂志》2013年第10期883-885,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：徐州市科技计划项目（NO.XZZD1225）

摘　　要：目的探讨芒果苷（Mangiterin）对慢性应激抑郁模型（chronicunpredictablestress，CUS）小鼠行为学的改善作用及对海马脑源性神经营养因子（brain—derivedneurotrophicfactor，BDNF）表达的影响。方法将大鼠随机分为正常对照组，慢性应激模型组，氟西汀组，芒果苷低、中和高剂量组，共6组，每组10只。采用慢性轻度不可预见性应激配合孤养复制抑郁模型，通过旷场及悬尾实验观察行为学改变，并运用免疫印迹检测海马BDNF的表达。结果与正常对照组比，模型组体质量增长缓慢[（5．33±1．20）g]，水平穿格次数减少[（102±18）~／5min]，活动距离缩短[（3425±112）mm]，悬尾不动时间延长，行为学明显处于抑郁状态，同时降低海马BDNF的表达（0．45±0．03），差异有统计学意义（P〈0．01）；而中药芒果苷组较模型组可显著改善行为学指标，体质量增长较快，升高海马BDNF的表达，差异有统计学意义（P〈0．01）。芒果苷高剂量组与氟西汀组比较，差异无统计学意义泸〉0．05）。结论芒果苷能够改善慢性应激抑郁模型小鼠的抑郁行为，可能与其上调海马BDNF的表达有关。Objective To explore the effects of mangiterin on behaviors and brain-derived neurotrophic factor（BDNF） of hippocampus in chronic stress depression mice. Methods 60 male mice were randomly divided into normol control group, model group, fluoxetin control group and mangiterin groups （low, medium and high dose）, 10 mice in each group. All mice except normal control group were singly housed and subjected to chronic stress-induced depression model for 21 consecutive days. The behaviors of mice were detected by open-field test and tail-suspension test. The expression of BDNF in the hippocampus were assessed using western blot. Results Compared with the normal group, mice exposed chronic stress showed decreased body weight （ （5.33 ＋ 1.20） g） , crossing lines （ 102 ＋ 18 ） and distances （ （ 3425 -＋ 112 ） mm） notably decreased in open-field behavior test. The duration of immobility during tail-suspension was increased significantly. BDNF expression in the hippo- campus （ 0.45 ＋ 0.03 ） was downregulated significantly（P〈 0. O1 ）, while it was upregulated in the groups of mangi- terin（P 〈 0.01 ）. Mangiterin group in high dose had the similar effects to fluoxetin group（P 〉 0.05 ）. Conclu- sion Mangiterin can ameliorate behavior impairment of chronic stress induced-depression mice and it may be re- lated to the upregulation of BDNF expression in the hippocampus.

关 键 词：芒果苷 慢性应激抑郁 脑源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]