培养时间、蔗糖质量浓度及^(60)Co-γ射线对木瓜花粉萌发的影响  

Influence of culturing time,mass concentration of sucrose and ^(60)Co-γ rays on germination of Chaenomeles sinensis pollen

作　　者：岳华峰[1] 唐丽丹[2] 马顺兴 任伟[2] 杨绍彬[1] 杨超伟[1] 袁晓雪[2] 李相宽 

机构地区：[1]国家林业局泡桐研究开发中心,河南郑州450003 [2]河南农业大学,河南郑州450002 [3]河南投资集团有限公司,河南郑州450008

出　　处：《河南农业大学学报》2013年第5期538-542,共5页Journal of Henan Agricultural University

基　　金：林业公益性行业科研专项(201204409);信阳市科技攻关项目(KJGG1205)

摘　　要：以木瓜花粉为试验材料,采用离体萌发培养法和60Co-γ射线辐射法,研究了花粉萌发的最佳检测时间,最佳蔗糖质量浓度及辐射育种的最适宜剂量.结果表明,木瓜花粉萌发最佳检测时间为6 h;随着蔗糖质量浓度的升高,花粉的萌发率呈先升高后下降的趋势,最佳蔗糖质量浓度为150 g·L-1;60Co-γ辐射处理极显著地影响了木瓜花粉的萌发率,且随着辐射剂量的增加,花粉萌发率呈显著下降趋势.通过萌发率拟合方程,得出木瓜花粉辐射的半致死剂量为254.14 Gy.Using the pollen of Chaenomeles sinensis as the test material, and adopting the germination in vitro culture method and 60Co-γ ray radiation, reseaches were conducted on the best detection time, the best sucrose mass concentration of pollen germination and the most appropriate dose of radiation breeding. The results are as follows, the best germination time for pollen of Chaenomeles was 6 h; with the sucrose mass concentration, pollen germination rate increased first and then decreased, the optimal sucrose mass concentration was 150 g· L-1; 60Co-γ radiation treatment significantly affected the Chaenomeles sinensis pollen germination rate moreover, with radiation dose increasing, the pollen germination rate showed a significant downward trend. The Chaenomeles sinensis pollen half-lethal dose of radiation was obtained as 254.14 Gy through the establishment of the fitting equation of the germination rate.

关 键 词：60Co-γ 木瓜花粉 蔗糖质量浓度 辐射效应 半致死剂量 

分 类 号：S603.2[农业科学—园艺学]