光学表面等离子共振生物传感器检测小麦转基因的研究  

Wheat transgene detection using optical surface plasmon resonance biosensor

作　　者：王婷婷[1,2] 李伟[3] 魏文松[1] 穆琳瑛[1] 胡建东[1,2] 

机构地区：[1]河南农业大学机电工程学院,河南郑州450002 [2]河南农大迅捷测试技术有限公司,河南郑州450002 [3]河南农业大学理学院,河南郑州450002

出　　处：《河南农业大学学报》2013年第5期580-583,共4页Journal of Henan Agricultural University

基　　金：河南省杰出青年基金项目(104100510017);河南省科技成果转化计划(农业)项目(112201110020);郑州市技术研究与开发经费支持项目(121PYFZX184)

摘　　要：采用在镀金芯片上修饰一层带巯基的DNA探针引物,构建了一种简单、快速、灵敏的光学表面等离子共振DNA生物传感检测系统.通过监测转基因片段探针引物与目的片段结合时响应信号的变化获得被检物质的浓度,建立了转基因检测标准曲线.最低检测限达0.27 mg·L-1.A simple, rapid and sensitive optical surface plasmon resonance based on DNA biological sensing detection system was constructed by modifying a layer of DNA probe primer attached to sulfydryl onto gold-plating chip. The concentration of transgene sample solution was obtained by monitoring the changes of the combining responding signal between the probe primer and the purpose fragment, in which the standard curve of transgene detection was established. The minimum limit of detection of 0.27 mg ·L-1 was obtained, which was less than one obtained by the agarose gel method.

关 键 词：表面等离子共振 生物传感芯片 探针引物 转基因片段 响应值 

分 类 号：TN29[电子电信—物理电子学]