雌激素对大鼠运动病易感性的影响  

Effect of estrogen on motion sickness susceptibility in rats

作　　者：葛新宇[1] 黄矛[1] 

机构地区：[1]第二军医大学基础医学部新药评价中心,上海200433

出　　处：《药学服务与研究》2013年第5期350-352,共3页Pharmaceutical Care and Research

基　　金：国家自然科学基金资助项目(81071613/H2101)

摘　　要：目的:研究雌激素对大鼠运动病易感性的影响。方法:将30只大鼠随机分为5组,均接受旋转刺激,建立运动病模型。5组大鼠在旋转前60min分别皮下注射同体积0.5ml/kg的生理盐水、6mg/ml的雌二醇、4mg/ml的雌激素α受体激动剂、4mg/ml的雌激素β受体激动剂,以及灌胃给予4mg/ml的他莫昔芬5ml/kg,以高岭土食用量和运动病评分作为衡量运动病反应程度的指标。用RT-PCR法检测大鼠在接受旋转刺激后,下丘脑中雌激素受体mRNA的表达量。结果:与生理盐水组相比,雌二醇组和雌激素α受体激动剂组大鼠接受旋转刺激后的高岭土食用量和运动病评分增加,运动病反应加重;他莫昔芬组大鼠的运动病反应减轻。接受旋转刺激的大鼠下丘脑中雌激素α受体mRNA表达量明显增加。结论:给予外源性雌激素会增加大鼠的运动病易感性,雌激素α受体可能在运动病反应中发挥主要作用。Objective：To investigate the effect of estrogen on motion sickness susceptibility in rats.Methods：Thirty rats were randomly divided into 5 groups.Motion sickness models were established by rotation stimulation.Sixty minutes before exposure to rotation stimulation,rats in the 4 groups were subcutanously injected with normal saline 0.5 ml/kg,6 mg/ml estradiol 0.5 ml/kg,4 mg/ml estrogen α receptor agonist （0.5 ml/kg） and 4 mg/ml estrogen β receptor agonist （0.5 ml/kg）,respectively.And rats in another group was gavaged with 4 mg/ml tamoxifen （5 ml/kg）.The intake of kaolin and the motion sickness scores were used as perimeters to evaluate the seriousness of motion sickness,and the expression of estrogen receptor mRNA in hypothalamus following rotation stimulation was determined by RT-PCR.Results：When compared with the normal saline group,the intake of kaolin and the motion sickness scores for the estradiol group and estrogen α receptor agonist group elevated considerably and symptoms of motion sickness were severer,while symptoms of motion sickness for the rats in the tamoxifen group were obviously lighter.The expression of estrogen α receptor mRNA in hypothalamus increased significantly following rotation stimulation.Conclusion：Administration of exogenous estrogen would aggravate motion sickness susceptibility,and estrogen α receptor might play an important role in the induction of motion sickness.

关 键 词：运动病 雌激素 雌激素受体 大鼠 

分 类 号：R977.12[医药卫生—药品]