深绿木霉产孢条件优化  

The Optimization Condition of Trichoderma atroviride Producing the Spore

作　　者：池玉杰[1] 伊洪伟[1] 艾志强[1] 韩树英[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《中国农学通报》2013年第28期42-45,共4页Chinese Agricultural Science Bulletin

基　　金：黑龙江省自然科学基金重点项目"防林木病害的长枝木霉菌抑菌活性物质结构鉴定和几丁质酶基因研究"(ZD200901)

摘　　要：为了获得深绿木霉最佳的产孢条件，通过测定不同条件下深绿木霉的产孢量，研究培养温度、初始pH、装液量和接菌量等因素对产孢的影响。结果表明：温度25℃、初始pH 6、装液量20 mL、初始接菌量0.1 mL（1.0×107 cfu/mL），培养7天时，产孢量较为理想。In order to obtain the optimization condition of Trichoderma atroviride producing the spore, the factors affecting the sporulation quantity, such as the cultivating temperature,the initial pH, the volume of medium and the volume of inoculum were investigated in present study. The results showed that, the sporulation quantity was best with 0.1 mL inoculum （1.0 × 107 cfu/mL） in the 20 mL medium under the initial pH 6 of 25℃for 7 days.

关 键 词：深绿木霉菌 产孢量 生物量 

分 类 号：S763.15[农业科学—森林保护学]