茶树硒营养代谢关键酶硒半胱氨酸甲基转移酶基因克隆及启动子结构分析  

The Gene Cloning About Selenocysteine Methyltransferase the Key Enzyme Related to Selenium Metabolism and the Structure Analysis of Its Promoter

作　　者：王雅楠[1] 陈晶晶[1] 朱林[2] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽农业大学资源与环境学院,合肥230036

出　　处：《中国农学通报》2013年第28期138-143,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"茶树硒营养代谢关键酶基因的表达及其调控机理研究"(30971870)

摘　　要：为探明茶树硒营养代谢关键酶基因硒半胱氨酸甲基转移酶(SMT,GenBank登录号:DQ480337)的表达调控规律,通过PCR和基因步移技术获得SMT的DNA全长和部分启动子序列,用PLACE、PlantCARE分析SMT内含子和启动子的序列,预测其顺式作用元件及功能。结果表明,SMT基因全长5473 bp,有7个外显子和6个内含子,内含子富含光响应元件、激素响应元件和抗逆性相关元件。通过基因步移法克隆得到的SMT启动子长375 bp,除了含核心启动子保守元件TATA-box和CAAT-box外,还有其他重要顺式作用元件,如光响应元件、低温响应元件和胚乳表达特异性元件等。植物硒营养代谢关键酶SMT启动子的克隆及结构分析为进一步揭示SMT基因的生物学功能和表达调控机制奠定了理论基础。In order to explore the regulation and control of gene expression about selenocysteine methyltransferase （SMT） the key enzyme related to selenium metabolism, the full-length DNA sequence and part promoter sequence of SMT were obtained using the technology of PCR and gene walking. The introns and promoter of SMT were analyzed by the tools of PLACE and PlantCARE to predicte its cis-acting element and function. The result showed that the full-length of SMT was 5473 bp which contained 7 exons and 6 introns. The introns had rich light responsive elements, hormone response element and resistance associated element. The promoter of SMT got by the method of genome walking was 375 bp. In addition to the core promoter conserved elements of TATA-box and CAAT-box, there are many other important cis-acting elements such as light response elements, low temperature response elements and endosperm specific expression elements and so on in the promoter. The promoter cloning and structural analyzing of key enzyme SMT gene related to selenium nutrition metabolism provided a theoretical basis for further revealing the biological function and regulation mechanism of SMTgene expression.

关 键 词：富硒茶(嫩叶) 硒半胱氨酸甲基转移酶 启动子 基因组步移 

分 类 号：Q291[生物学—细胞生物学]