无籽罗汉果组培无菌系的构建  

The Construction of the Aseptic Strain of the Tissue Culture of Seedless Siraitia grosvenorii

作　　者：潘丽梅 莫长明 马小军[2] 冯世鑫 唐其 白隆华 韦荣昌 邢爱佳[3] 

机构地区：[1]中国医学科学院-北京协和医学院药用植物研究所广西分所,南宁530023 [2]中国医学科学院-北京协和医学院药用植物研究所,北京100193 [3]广西大学,南宁530004

出　　处：《中国农学通报》2013年第28期150-155,共6页Chinese Agricultural Science Bulletin

基　　金：国家十二五科技支撑计划项目"罗汉果质量改进和综合开发利用"(2011BAI01B03);国家科技重大专项子课题"罗汉果;佛手;青蒿种子(苗)质量标准研究"(2012ZX09304006);广西科学研究与技术开发研究项目"无籽罗汉果产量和品种促控技术研究"(桂科攻11107010);广西研究生科研创新项目"无籽罗汉果遗传转化体系的构建"(T32560)

摘　　要：研究无籽罗汉果组培快繁技术要点,为无籽罗汉果再生体系建立和工厂化生产提供技术支持。以幼嫩茎段为试验材料,探讨不同灭菌时间,不同激素组合以及浓度对启动培养、增殖培养、生根培养等的影响。无籽罗汉果带芽茎段最佳消毒方法为1%HgCl_2溶液消毒8 min。最适宜的无籽罗汉果茎段启动培养基为MS+1.0 mg/L 6-BA+1.5 mg/L KT,无籽罗汉果继代增殖培养基为MS+1.0 mg/L 6-BA+0.5 mg/L IBA,增殖系数可达6.8;较适合生根的培养基为1/2MS+1.0 mg/L IBA+0.5 mg/L NAA,新稍平均生根数达7.4条,生根率可达95%。可为无籽罗汉果大规模组织培养和快速繁殖提供理论基础,并为组织培养高效体系的建立及外源基因导入提供参考。This research explored the tissue culture and rapid propagation of seedless Siraitia grosvenorii in order to provide technical supports on seedless Siraitia grosvenorii regenerations system establishment and factory production. The young stem of seedless Siraitia grosvenorii was used as explants to investigate the effects of different sterilization time, different hormone combinations and concentrations on the initiating, proliferation, as well as rooting cluster buds were studied. The results showed that the best sterilization method for the stem segments was agitating in 0.1% HgCl2 for 8 min. The optimum medium for initiation culture of stem segments was MS＋1.0 mg/L 6-BA＋1.5 mg/L KT. The suitable multiplication medium was MS＋1.0 mg/L 6-BA＋0.5 mg/L IBA, with the bud proliferation coefficient of 6.8. The suitable rooting medium was 1/2MS＋1.0 mg/L IBA＋0.5 mg/L NAA, in which the average root number was 7.4, the rooting rate of multiplied buds was up to 95%. It could provide theoretical basis for the large-scale production of the tissue culture and rapid propagation of seedless Siraitia grosvenorii, and the experiment provided references for the establishment of efficient tissue culture regeneration system and the exogenous gene transduction.

关 键 词：无籽罗汉果 激素 组织培养 

分 类 号：Q813.1[生物学—生物工程]