外源性孕酮对牛卵母细胞体外核成熟的影响  

The Effect of Exogenous Progesterone on in vitro Nuclear Maturation of Bovine Oocytes

作　　者：杨菲[1] 姚学军 闫芃[1] 梁琳[1] 郑雪莹[1] 邢书涵[1] 刘云海[1] 郭勇[1] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京昌平区动物疫病预防与控制中心,北京102200

出　　处：《中国农学通报》2013年第29期42-45,共4页Chinese Agricultural Science Bulletin

基　　金：2011年度国家自然基金面上项目"不同来源植入前期绵羊胚胎IFNτ差异表达及其体外诱导子宫上皮细胞Galectin15表达的研究"(31072185);2013年度国家自然基金面上项目"牛体内外植入前胚胎IFNτ的差异表达及对子宫上皮细胞ISG15和Wnt7a表达的体外诱导(31272526)

摘　　要：为了研究外源性孕酮对牛卵母细胞体外核成熟的作用,通过向成熟培养液中添加10μg/mL FSH＋10μg/mL LH（对照组）和0、10、100、1000 ng/mL的外源性孕酮,对牛卵丘卵母细胞复合体进行体外成熟培养,在培养8 h后用醋酸地衣红染色观察各自的生发泡破裂率,然后在成熟22 h统计第一极体率,体外受精后7~8天统计囊胚率。结果表明：对照组卵母细胞的生发泡破裂率（75.0%）和极体率（79.6%）均明显高于添加不同浓度孕酮的各处理组（P〈0.05）,而囊胚率各组之间均无显著差异（P〉0.05）。因此,外源性孕酮不能促进牛卵母细胞的核成熟,单独添加外源性孕酮不能起到与内源性孕酮相同的作用。In this experiment,it was aimed to investigate the effect of exogenous progesterone on in vitro nuclear maturation of bovine oocytes through adding with 10 μg/mL FSH ＋ 10 μg/mL LH（control）,0,10,100,1000 ng/mL exogenous progesterone respectively into the in vitro maturation medium for bovine cumulus-oocyte complexes（COCs）.The germinal vesicle breakdown（GVBD） rate of each group after 8 h using acetic acid-orcein staining,and counted first polarbody（PbI） rate after 22 h were all analyzed.Finally,the blastocyst rate after 7-8 days of in vitro fertilization was also counted.The results showed that,GVBD（75.0%） and PbI（79.6%） rate of the control group were significantly higher than the other ones adding with exogenous progesterone（P＆lt;0.05）,and blastocyst rate of each group didn’ t show significant variation（P＆gt;0.05）.Therefore,it could be concluded that the exogenous progesterone could not play a role for improving the nuclear maturation of bovine oocytes,it also meant that exogenous progesterone alone could not take the same effect as endogenous progesterone in such kind of processes.

关 键 词：牛 孕酮 卵母细胞 体外成熟 

分 类 号：S823.3[农业科学—畜牧学]