NaCl胁迫对留兰香基因组DNA及其甲基化的影响  

The Effects of Genomic DNA and DNA Methylation of Menthae spicatae L. with Salt Stress

作　　者：李忠爱[1] 郭强梨[1] 王子成[1] 

机构地区：[1]河南大学生命科学学院植物种质资源与遗传工程试验室,河南开封475004

出　　处：《中国农学通报》2013年第30期159-165,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"表观遗传调控菊花花期及其机制解析"(31171982)

摘　　要：研究了NaCl处理对留兰香生长发育和生理生化反应的影响,以及对基因组DNA及其甲基化的影响。将留兰香组培苗接种于添加了NaCl的MS培养基上(NaCl浓度分别为0,50、100、150 mmol/L),用混合取样法,取同一生长时期植株嫩叶,提取植物基因组DNA,用AFLP,MSAP的方法进行相关的分析。结果表明,NaCl处理20天后,随着盐浓度的升高,叶绿素的含量逐渐降低,MDA和脯氨酸含量逐渐增大。AFLP分析表明,处理组与对照组之间未发现特异片段,基因组序列未发生变异。MSAP分析表明,50 mmol/L NaCl处理会引起全基因组DNA的甲基化水平降低,而100、150 mmol/L NaCl则诱导了全基因组DNA的甲基化水平升高;与对照相比,50、100、150 mmol/L NaCl处理后DNA甲基化和去甲基化比率分别为4.35%、9.93%、12.46%和12.73%、13.12%、20.54%。NaCl可影响留兰香的生长发育,引起各项生理生化指标的改变。NaCl虽然不造成留兰香基因序列的变异,但是可以引起其基因组DNA甲基化水平的改变。低浓度的NaCl引起基因组DNA甲基化水平降低,高浓度的NaCl可引起基因组DNA甲基化水平升高。The influence of salinity on spearmint growth and physiological and biochemical reactions, and the effects on genomic DNA and DNA methylation. Spearmint seedlings were grown on MS medium supplied with NaCI （0, 50, 100 and 150 mmol/L, respectively） for 20 days. Genomic DNA was extracted from leaves. The amplified fragment length polymorphisms （AFLP） and methylation sensitive amplification polymorphism （MSAP） was used to study the genetic and epigenetic stability. The chlorophyll content was significantly reduced, whereas the MDA and Pro content increased as the concentration of salt increased when the Mentbae spieatae L. seedlings were treated by NaCl for 20 days. AFLP technique did not detect any sequence variation; this meant that there was no difference in the genomic DNA polymorphism existed. The level of DNA methylation was reduced with concentrations of 50 mmol/L NaCl treatment and increased with concentrations of 100 and 150 mmol/L NaCI treatment; the ratio of methylation and demethylation of genomic DNA of the seedlings treated with 50, 100, and 150 mmol/L NaCl was 4.35%, 9.93%, 12.46% and 12.73%, 13.12%, 20.54%, respectively. The growth and physiological and biochemical reactions of Menthae spicatae L. had changed with NaCl treatment. Salt could not impact the genomic DNA sequence; however, it could result in the variation of DNA methylation.

关 键 词：留兰香 DNA甲基化 NACL AFLP MSAP 

分 类 号：Q945.78[生物学—植物学]