陆地棉遗传背景下海岛棉Chromsome7片段纤维品质性状的QTL定位  

QTL Mapping of Fiber Quality Traits in Gossypium hirsutum contributed by Introgressive Segments of Chromosome 7 from Gossypium barbadense

作　　者：马和欢[1,2] 王芙蓉[2] 刘国栋[2] 高阳[1,2] 张传云[2] 张军[1,2] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东棉花研究中心/农业部黄淮海棉花遗传改良与栽培生理重点实验室,山东济南250100

出　　处：《山东农业科学》2013年第10期7-11,共5页Shandong Agricultural Sciences

基　　金：国家自然基金项目(31171598);国家"863"计划项目(2012AA101108)

摘　　要：为消除遗传背景的干扰,深入剖析陆地棉遗传背景下渗入的海岛棉Chromsome 7(Chr.7)片段对优异纤维性状的遗传贡献,本研究从(鲁棉研22×鲁原343)重组自交系中选择携带海岛棉Chr.7染色体片段的优质次级渐渗系R472作亲本,与高产亲本鲁棉研22回交,构建了包含514个F2单株的次级定位群体。利用JoinMap构建Chr.7的遗传图谱,运用基于混合线性模型的QTLNetwork-2.2软件和基于多元回归模型的Windows QTL Cartographer 2.5软件对纤维品质性状进行QTL定位,结果显示,利用Windows QTL Cartographer 2.5检测到1个纤维强力QTL(qFS-C7-1),距离渐渗位点DC40182 8.1 cM,可解释表型变异率5.66%,增效等位基因来源于携带有海岛棉Chr.7染色体渐渗片段的R472;利用QTLNetwork-2.2检测到1个纤维强力QTL(qFS-C7-1)、1个纤维细度QTL(qFM-C7-1)。2个作图软件定位的纤维强力QTL位点基本一致,可认为是同一位点。In order to analyze the genetic contribution of introgressive segments of chromosome 7 （ Chr. 7） to fiber traits, a secondary mapping population including 514 individuals was developed from a cross between secondary introgression line （SIL） R472 containing chromosomal segment of Chr. 7 from Gossypium barbadense, and recurrent parent LMY22. The linkage map was constructed using JoinMap, and the QTL identification was performed by QTLNetwork - 2.2 and composite interval mapping approach of Windows QTL Cartographer 2.5. The results showed that one QTL for fiber strength （ qFS - C7 - 1 ） was detected by Windows QTL Cartographer 2.5, which had the distance to the site of DC40182 as 8.1 cM, and explaining 5.66% of the phenotypic variance; the favorable alleles were derived from the R472 parent. One QTL for fiber strength （ qFS - C7 - 1 ） and one for fiber fineness （ qFM - C7 - 1 ） were detected by QTLNetwork - 2.2. The QTLs for fiber strength detected by the two mapping software were located in the same interval.

关 键 词：海岛棉 次级渐渗系 纤维品质 QTL定位 

分 类 号：S562.032[农业科学—作物学]