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机构地区:[1]广西中医药大学附属瑞康医院,广西530011
出 处:《北京中医药大学学报》2013年第10期692-695,共4页Journal of Beijing University of Traditional Chinese Medicine
基 金:广西中医药科技专项资助(No.GZKZ10-061)
摘 要:目的观察穴位埋线对非酒精性脂肪肝大鼠肝脏组织胰岛素JNK1信号通路的调控作用。方法采用高脂饮食喂养雄性SD大鼠12周,建立非酒精性脂肪肝大鼠模型。以穴位埋线治疗8周后,对比模型组、埋线组肝指数变化,并用Western blot方法,检测穴位埋线前后肝脏组织中胰岛素受体(IR)、磷酸化胰岛素受体底物1(p-IRS1,Ser307位点)、C-Jun氨基端激酶1(JNK1)及磷酸化C-Jun氨基端激酶1(1p-JNK1)表达的变化。结果穴位埋线组大鼠肝指教较模型组明显降低(P<0.05);穴位埋线组肝脏组织IR较模型组无明显变化(P<0.05),p-IRS1、JNK1及p-JNK1表达水平明显低于模型组(P<0.05)。结论穴位埋线对非酒精性脂肪肝有治疗作用,其作用机制可能与降低JNK1的表达以及抑制JNK1的磷酸化,并进一步抑制其Ser307位点的磷酸化,从而改善胰岛素抵抗有关。Objective To observe the regulatory effect of acupoint catgut embedding therapy on liver insulin JNK1 signal pathway in rats with nonalcoholic fatty liver.Methods Male SD rats were feed with fatty food for 12 weeks for establishing rat model of nonalcoholic fatty liver.After treatment with cupoint catgut embedding therapy for 8 weeks,the changes of liver index were compared between model group and embedding group.The expression changes of liver insulin receptor,phosphorylation of insulin receptor substrate 1 (p-IRS1,Ser307 loci),C-Jun N-terminal kinase 1 (JNK1) and C-phosphorylation of Jun N-terminal kinase 1 (p-JNK1) were detected by using Western blot method before and after treatment.The mechanism of cupoint catgut embedding therapy in influencing JNK1 expression and activating and regulating main signal molecules of insulin pathway was analyzed.Results The liver index was significantly lower in embedding group than that in model group (P < 0.05).The changes of liver insulin receptor were not significant (P < 0.05),and the expressions of pVIRS1,JNK1 and p-JNK1 were significantly lower in embedding group than those in model group (P < 0.05).Conclusion Acupoint catgut embedding therapy has therapeutic effect on nonalcoholic fatty liver.The mechanism may be related to that it can decrease JNK1 expression,inhibit phosporylation of JNK1 and further inhibit the phosporylation of Ser307,and relieve insulin resistance.
关 键 词:C-JUN氨基端激酶 非酒精性脂肪肝病 胰岛素抵抗 穴位埋线 大鼠
分 类 号:R245.91[医药卫生—针灸推拿学]
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