甘草次酸-丹参酮ⅡA复方脂质体包封率测定方法研究  

Determination method for entrapment efficiency of glycyrrhetinic acidtanshinone IIA compound liposomes

作　　者：林珈好[1] 王秀丽[1] 曹唯仪[1] 黄镇林[1] 王玉蓉[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2013年第10期701-704,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81202928);北京市自然科学基金资助项目(No.7123118)

摘　　要：目的建立甘草次酸-丹参酮ⅡA复方脂质体(GT-Lip)包封率的测定方法。方法分别采用葡聚糖凝胶过滤法、低速离心法分离脂质体和游离药物。以HPLC法为分析手段,比较该2种方法包封率的测定结果。结果葡聚糖凝胶G-50(M)对脂质体有一定吸附,不适合GT-Lip包封率的测定;低速离心法能使GT-Lip脂质体与游离药物有效分离,经HPLC法测定,丹参酮ⅡA(TSN)与甘草次酸(GA)平均包封率分别达到(81.50±0.76)%和(98.63±0.90)%。结论 HPLC法与低速离心法相结合可快速、有效用于甘草次酸-丹参酮ⅡA复方脂质体的包封率测定。Objective To develop a method for determining the entrapment efficiency of glycyrrhetinic acid-tanshinone IIA compound liposomes （GT-Lip）.Methods Sephadex filtration and low-speed centrifugation were used for separating GT-Lip and free drugs.The determination results of these two methods for the entrapment efficiency were compared taken HPLC as analysis approach.Results Sephadex filtration G-50 （M） absorbed some GT-Lip,which was not good for the determination of the entrapment efficiency of GT-Lip.The low-speed centrifugation separated effectively GT-Lip and free drugs,and the average entrapment efficiency of tanshinone IIA reached to （81.50 ± 0.76） ％ and that of glycyrrhetinic acid reached to （98.63 ± 0.90） ％ after HPLC determination.Conclusion HPLC combining low-speed centrifugation can applied for the effective determination of the entrapment efficiency of GT-Lip,which is quick and accurate.

关 键 词：甘草次酸 丹参酮ⅡA 包封率 低速离心法 葡聚糖凝胶过滤法 

分 类 号：R283.4[医药卫生—中药学]