孝感地区RhD阴性个体分子生物学特征初步研究  

Molecular Biological Characteristics of RhD-negative Individuals in Xiaogan Area of Hubei Province,China

作　　者：戈勇[1] 涂同涛[1] 柯秋高 邓曦 柯卫泽 栾玲峰 魏晴 

机构地区：[1]华中科技大学同济医学院附属同济医院输血科,武汉430030 [2]孝感市中心血站,孝感462000

出　　处：《华中科技大学学报（医学版）》2013年第5期585-587,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：2008年湖北省卫生厅血液专项基金资助项目(No.CGX2008-6)

摘　　要：目的分析孝感地区RhD阴性个体的分子生物学特征。方法对血清学检测为RhD阴性的标本,通过PCRSSP的方法对RHD特异性外显子7及内含子4,启动子及外显子10进行筛查。阳性标本进一步筛查RHD基因特异性外显子3、4、5、6、7、9及RHD1227A,即DEL。结果在94例RhD阴性标本中,66.0%(62/94)为RHD基因完全缺失,23.4%(22/94)含完整的RHD基因,其中21例为RHD1227A,占22.3%(21/94),10.6%(10/94)为RHD基因部分缺失。结论 RHD基因缺失为孝感地区RhD阴性个体的主要分子生物学特征,DEL为次主要分子生物学特征。Objective To examine the molecular biological characteristics of RhD-negative individuals in Xiaogan area of Hubei province,China. Methods RhD-negative blood samples were obtained and serologically detected. PCR-SSP was used to detect RHD exon 7 and intron 4 in these samples. With samples negative for RHD exon 7 and intron 4, RHD exon 10 and the promoter of the samples were further detected. With samples positive for RHD exon 7 and intron 4, RHD exons 3,4,5,6,7,9 and RHD1227A were determined. Results Complete deletion of RHD gene was found in 62 cases out of all the 94 RhD-negative samples （66%）. Complete RHD gene was observed in 22 cases （23.4%） ,among which 21 were RHD1227A （DEL）. Ten cases had partial deletion of RHD gene. Conclusion Complete deletion of RHD gene is the main molecular biological characteristics of RhD-negative individuals in Xiaogan area. DEL ranks second in these characteristics.

关 键 词：RHD阴性 RHD1227A 序列特异性引物聚合酶链反应 

分 类 号：R446.11[医药卫生—诊断学]