烟酸和普罗布考联合应用对巨噬细胞胆固醇流出的影响  

Effect of Niacin Combined with Probucol on Cholesterol Efflux in Macrophage and the Possible Mechanism

作　　者：董静[1] 施文剑[2] 赵水平[3] 

机构地区：[1]湖南省肿瘤医院重症医学科,湖南长沙410006 [2]湖南省人民医院肾内科,湖南长沙410005 [3]中南大学湘雅二医院心内科,湖南长沙410011

出　　处：《医学临床研究》2013年第10期1882-1886,共5页Journal of Clinical Research

摘　　要：【目的】探讨烟酸、普罗布考单独及联合作用对巨噬细胞胆固醇流出的影响及其机制。【方法】巨噬细胞264．7分别给予烟酸（1．0mM）、普罗布考（50μM）、烟酸加普罗布考（1．0mM＋50μM）干预24h后，收集细胞，反转录聚合酶链反应测定巨噬细胞B族清道夫受体I（SR-BI）、三磷酸腺苷结合盒转运A1（ABCAl）和三磷酸腺苷结合盒转运体G1（ABCGl）mRNA表达，采用液体闪烁计数器检测细胞内胆固醇流出情况。【结果】与对照组比较，烟酸和普罗布考单独作用及联合应用均促进巨噬细胞胆固醇流出，但联合作用组与单独作用组之间效果差异无显著性（P〉0．05）。烟酸及普罗布考对巨噬细胞SR-BI mRNA的表达无影响；对ABCA1 mRNA的表达有相反作用；二者均增加巨噬细胞ABCG1 mRNA的表达，并促进其介导的胆固醇流出。【结论】烟酸和普罗布考联合应用可促进巨噬细胞胆固醇流出；ABCG1-HDL途径在胆固醇流出中占有更重要的地位，这可能为解释烟酸和普罗布考抗动脉粥样硬化的机制提供有用的线索。[Objective] To explore the effect of niacin or probucol alone and their combination on cholesterol efflux in macrophage and the possible mechanism. [Methods] Macrophage 264.7 cells were interfered with niacin（1.0mM）, probucol（50μM）, niacin combined with probucol（1.0mM ＋ 50μM） for 24h, and then the cells were collected. Reverse transcription polymerase chain reaction（RT PCR） was used to determine mRNA expression of B-group scavenger receptor I（SR-Bi）, adenosine tripho.sphate-binding cassette transporter A1 （ABCA1） and adenosine triphosphate-hinding cassette transporter GI （ABCG1） in macrophage. Liquid scintillation counter was used to detect cholesterol efflux. [Results]Compared with the control group, niacin or probucol alone and their combination could promote cholesterol efflux in maerophages, but there was no significant difference in the efficacy between alone group and combination group. Niacin and probucol did not affect the mRNA expression of SR BI, but had the contradictory effect on the expression of ABCA1, and both of them increased mRNA expression of ABCG1 in maerophages and promoted ABCOl-mediated cholesterol efflux.[ConclusionlNiacin combined with probucol can promote cholesterol efflux in macrophage. ABCG1-HDL pathway plays an important role in cholesterol efflux, which may provide the useful clue for explaining the mechanism of anti-atherosclerosis of niacin and probucol.

关 键 词：巨噬细胞 胆固醇 尼克酸 普罗布可 

分 类 号：R589.22[医药卫生—内分泌]