不同激发方式对大鼠哮喘模型的影响  

Influence of different challenge methods to animal model with bronchial asthma in rats

作　　者：龚萍[1] 李云[1] 钟礼立[1] 李文开[1] 刘持[2] 

机构地区：[1]湖南省人民医院,湖南长沙410005 [2]中南大学湘雅三医院,湖南长沙410013

出　　处：《医学临床研究》2013年第10期1979-1982,共4页Journal of Clinical Research

摘　　要：【目的】探讨泵雾化和超声雾化两种激发方式制备大鼠哮喘模型的优劣。【方法】将24只SD大鼠分为三组：对照组、超声雾化组和泵雾化组，每组各8只。对照组予1mL生理盐水腹腔注射，其余两组予10％卵蛋白（0VA）混合液1mL致敏。2周后再以0VA进行激发，泵雾化组用高频雾化器雾化吸入2％0VA进行激发，超声雾化组用超声雾化器进行激发，对照组以生理盐水激发。各组实验大鼠均采用BUXCO测定气道阻力；取右肺组织用于HE、Masson和AB_PAs染色，分析气道炎症和气道重塑情况；做左肺支气管肺泡灌洗（BAL），其灌洗液（BALF）行细胞学分析。【结果】①激发结束时，超声雾化组和泵雾化组大鼠均出现呼吸急促、点头状呼吸、腹肌痉挛等表现，而对照组大鼠激发前后无明显变化，呼吸平稳，运动敏捷。与对照组比较，泵雾化组和超声雾化组支气管周围炎性细胞浸润评分、支气管管壁周围嗜酸性粒细胞（EOS）显著增高（P〈0．01）；泵雾化组与超声雾化组无显著差异（P〉0．05）；②与对照组比较，泵雾化组与超声雾化组气道平滑肌面积、杯状细胞占上皮细胞百分比以及胶原沉积面积均明显增高（P〈0．01）；泵雾化组较超声雾化组更高（P〈0．01）；③相对于对照组，泵雾化组及超声雾化组BALF细胞总数、各细胞分类中EOS，淋巴细胞显著增高（P〈0．01）；但泵雾化组与超声雾化组无显著差异（P〉0．05）；④超声雾化组及泵雾化组大鼠Raw值均大于对照组（P〈0．01）；泵雾化组大鼠Raw值较超声雾化组明显增高（P〈0．05）。【结论】①0VA超声雾化或泵雾化激发均能够成功复制哮喘大鼠模型。②使用改良后泵雾化激发的方式复制哮喘大鼠模型优于传统的超声雾化激发方式。[Objective]To establish the rat asthma model through pump and ultrasonic atomization, and explore difference of two ways . [Methods]Twenty-four male SD rats were randomly divided equally into three groups. The rats of control group inhaled 0.9 % sodium chloride through Ultra method in the challenge stage. The rats in Ultra group inhaled 2% OVA by Ultra atomizer;And The rats in the Pump group inhaled 2% OVA by Pump Atomization. All the rats of the different groups were detected by pulmonary function. The left lobe of the lung bronchoalveolar lavage（BAL） was performed to evaluate the airway inflammation. After BAL, the right lobe of the lung was removed for histological examination and were stained with HE, Masson and PAS. [Results]l. General observation of the animals Rats in Ultra group and pump group appeared appeared visible respiratory distress signs. However, rats in control group were almost the same： breathing steadly, moving swiftly. 2.1）The score of inflammatory cell infiltration, EOS around bronchus wall in ultra group and pump group were higher compared with control group （ P 〈0.01） ;That was not statistically different between ultra group , pump group （ P 〉0.05）. 2）Collagen deposition and goblet cells proliferation, mucus secretion were significantly increased in Ultra group and pump group compared with control;And these parameters in pump group were higher than those in ultra group （ P 〈0.01）. 3. The number of BALF cells ,cell percentage of EOS and lymphocyte in Ultra group and pump group was significantly increased compared with control group（ P 〈0. 01） ; however, there was no statistically significant difference between ultra group and pump group （ P 〉0.05）. 4. Raw value in pump group higher than that of Ultra group（ P 〈0.05）. [Conclusion]Inhaling OVA with pump and Ultra methods to challenge established the rat asthma model successfully. The pump method is superior to the traditional Ultra method to challenge the rat asthma model.

关 键 词：哮喘 疾病模型  动物 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]