传染性囊病病毒感染对SPF雏鸡免疫器官免疫功能的影响  

Influence of infectious bursal disease virus on immune function of immune organs of SPF chicks

作　　者：王灵娟[1] 高雪丽[1] 隋欣[1] 于志强[1] 李博韬[1] 郑世民[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2013年第10期11-13,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金青年基金(30901062)

摘　　要：以21日龄SPF雏鸡为研究对象,应用细胞培养和MTT及免疫组织化学方法分别检测了免疫器官中T、B淋巴细胞增殖功能和IgG、IgA、IgM生成细胞数量的动态变化,旨在揭示传染性囊病病毒(IBDV)感染对雏鸡免疫器官细胞免疫和体液免疫功能的影响。结果显示,IBDV感染雏鸡免疫器官中T、B淋巴细胞增殖功能和腔上囊、脾脏中IgG、IgA、IgM生成细胞数量不同程度的低于对照雏鸡,并且在感染后期逐渐恢复。表明雏鸡感染IBDV后的早期,机体免疫器官的细胞免疫和体液免疫功能均受抑制,可能是病毒感染早期导致雏鸡死亡的一个主要因素。In this experiment, cell culture. MTT and immunohistochemistry were used to examine the changes of T and B lymphocytes proliferation （IBDV）. The results showed that the T、B lymphocytes proliferation ability in immune organs were lower than control groups at fist stage,all those in the later stage of the disease begin to recover and immune re-sistance in the later stage. Experimental investigation showed that the cellular and humoral immunity were lower than that in control groups which were the important factors to lead to the dead of the infectious chicks.

关 键 词：IBDV 免疫器官 细胞免疫 体液免疫 

分 类 号：S858.31[农业科学—临床兽医学]