玉米耐盐种质筛选及群体遗传结构分析  

Screening of Maize Germplasms for Salt-tolerance and Evaluation of Population Genetic Structure

作　　者：郝德荣[1] 程玉静[1] 徐辰武 冒宇翔[1] 彭长俊[1] 薛林[1] 

机构地区：[1]江苏沿江地区农业科学研究所,南通226541 [2]扬州大学作物遗传生理省级重点实验室,扬州225009

出　　处：《植物遗传资源学报》2013年第6期1153-1160,共8页Journal of Plant Genetic Resources

基　　金：江苏省农业科技自主创新资金项目[CX(12)3067];南通市科技平台建设项目(CP12012002);南通市应用研究计划(BK2012084)

摘　　要：采用盆栽法评价了157份玉米自交系的苗期耐盐性,并利用115对SSR标记解析了耐盐自交系的群体遗传结构。结果表明,T5V、N1026、农大1145及4S等10份玉米自交系为高耐盐玉米种质;处理后10 d株高、地上鲜重、地下鲜重、地上干重、地下干重及存活率可作为玉米苗期耐盐鉴定的重要指标;利用SSR分子标记,结合系谱资料,将157份自交系划为6个类群,其中具有通系5血缘(类群Ⅰ)、泰国糯玉米种质血缘(类群Ⅲ)及旅大红骨、黄早四等血缘(类群Ⅵ)的自交系耐盐性较强,是开展玉米耐盐育种的重要种质类群。本研究筛选到的耐盐种质将为玉米耐盐遗传机制研究、玉米种质遗传改良及耐盐分子育种提供优异的基因资源。The salt tolerances of 157 maize inbred lines were evaluated during the seedling stage by means of potted identification, and the population structure of the studied inbred lines was analyzed using 115 SSR markers. The results showed that ten maize inbred lines, including T5 V, N1026, N ongda 1145,4S and so on, were highly sah- tolerant maize germplasms and the plant height after treatment for 10 （10DPH） , shoot fresh weight （SFW） , root fresh weight （RFW）, shoot dry weight （SDW）, root dry weight （RDW）, and survival rate （SR） could be used as key indicators for identification of salt tolerance in the maize seedling stage. Evaluation of population structure indi- cated that 157 inbred lines could be classified into six groups, which were generally consistent with their known ped- igrees. The inbred lines derived from Tongxi 5 （ group I ）, the waxy germplasms from Thailand （ group III ） and those from LRC,Huangzaosi（ group VI）and so on,were highly tolerant to salt,which were important germpalsms in maize breeding for salt tolerance. These screened salt-tolerant maize germplasms would provide excellent genetic re- sources for the studies of genetic mechanisms in maize, maize improvement, and molecular breeding for salt tolerance in the future.

关 键 词：玉米 耐盐性 评价 SSR标记 遗传结构 

分 类 号：S513[农业科学—作物学]