抗花叶病烟草种质资源的鉴定与筛选  

Selection and Identification of Tobacco Geramplasms Resistance Ability to TMV

作　　者：林龙云[1] 张燕云[2] 周以飞[2] 程崖芝[3] 郭金平[3] 潘大仁[1] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建农林大学作物科学学院,福州350002 [3]福建省烟草公司,福州350001

出　　处：《植物遗传资源学报》2013年第6期1173-1178,共6页Journal of Plant Genetic Resources

基　　金：福建省自然科学基金项目(2010J01070);福建省烟草专卖局资助项目(闽烟科2008(13)号)

摘　　要：以不同来源、不同类型的46个烟草品种为试验材料,进行抗烟草普通花叶病(TMV)特性的田间鉴定和分子鉴定,为筛选抗病烟草种质奠定基础。结果表明,22个品种的烟草抗TMV特性田间鉴定结果与前人鉴定结果基本相同,从24个前人未鉴定的烟草品种中初步筛选出14个中抗品种,8个中感品种和2个感病品种;通过抗TMV基因(CN)的特异引物进行扩增,35个烟草品种的基因组DNA具有特异性片段;通过分子鉴定和田间鉴定的结果比较,在具有CN或其同源基因35个烟草品种中62.9%田间鉴定表现为抗或中抗,83.3%的具有CN或其同源基因的选育烤烟品种田间鉴定表现为抗或中抗;结合两种方法,初步确定了3个中感品种和11个中抗品种。In this study,46 different types and sources of tobacco germplasms were selected as experiment ma- terials to screen disease resistant tobacco varieties through field investigation and molecular identification of resist- ance ability to TMV. The results of field investigation showed that 14 moderate resistant tobacco germplasms,8 mod- erate susceptible germplasms,and 2 susceptible ones were sereened out from 24 unidentified tobacco geramplasms, while 22 tobacco geramplasms were resistant to TMV which basically the same as previous results. Furthermore,spe- cific fragments were amplified in 35 tobacco genomic DNA by PCR method of specific primers of TMV-resistant gene （CN）. The results showed that 62.9% of 35 tobacco germplasms and 83.3% of bred varieties which had CN gene or homologous gene were resistant or moderate resistant to TMV by field investigation and molecular identifica- tion. Finally,3 moderate susceptible and 11 moderate resistant tobacco geramplasms were identified by combining both the above two methods.

关 键 词：烟草 TMV 抗花叶病 鉴定 

分 类 号：S572[农业科学—烟草工业]