白藜芦醇抗呼吸道合胞病毒作用体外实验研究  

Study on the effect of resveratrol against RSV in vitro

作　　者：谢秀春 徐建亚[1] 汪受传[1] 

机构地区：[1]南京中医药大学,南京210029

出　　处：《中华中医药杂志》2013年第11期3182-3184,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81072840);高等学校博士学科点专项科研基金(No.20103237110003)~~

摘　　要：目的:通过体外实验探讨白藜芦醇抗呼吸道合胞病毒(RSV)作用。方法:将RSV接种在人喉癌上皮细胞(Hep-2)上,采用细胞病变效应(CPE)和MTT法,以利巴韦林作为阳性对照药物,观察白藜芦醇在治疗、预防、直接抑制3种作用模式下细胞存活率。结果:3种作用模式对RSV均有一定程度的抑制作用,且治疗作用和预防作用较明显,在预防作用模式下,白藜芦醇组CPE与利巴韦林组相比明显减少;治疗作用模式下,与RSV组相比,白藜芦醇组对病毒的抑制率较高(P<0.05)。结论:白藜芦醇对RSV具有一定的体外抑制作用。Objective： To investigate the effect of resveratrol against respiratory syncytial virus （RSV） through experiments of in vitro. Methods： The cell survival rates of resveratrol in the treatment, prevention, direct inhibition of three kinds of mode were measured by cytopathic effect reduction assay and MTT assay, when Hep-2 cell was infected with RSV and the antiviral effect of resveratrol with ribavidn as the positive control drug. Results： The three of actions have a certain degree of inhibition, therapeutic effect and preventive effect, which is more pronounced. In preventive effect mode, the CPE was typically reduced in resveratrol group compared with ribavidn group; in therapeutic effect mode, the virus inhibition rate of resveratrol group was higher compared with RSV infected group （P〈0.05）. Conclusion： Resveratrol may have some protection against RSV in vitro.

关 键 词：白藜芦醇 呼吸道合胞病毒 体外 

分 类 号：R285.5[医药卫生—中药学]