逆灸命门穴对力竭大鼠糖原、ghrelin水平的影响  

Effects of preventive moxibustion at Mingmen(DU-4) on glycogen and ghrelin of exhaustive swimming rats

作　　者：孙志芳[1] 李晓泓[2] 王婧[3] 莫捷[2] 何龙[4] 丁娜[5] 朱世鹏[2] 张露芬[2] 

机构地区：[1]杭州师范大学医学部,杭州310036 [2]北京中医药大学,北京100029 [3]中国中医科学院中医临床基础医学研究所,北京100700 [4]中国中医科学院望京医院,北京100102 [5]扬州市妇幼保健院,扬州225002

出　　处：《中华中医药杂志》2013年第11期3193-3195,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金课题(No.90209026);国家中医药管理局针灸特色疗法评价重点研究室项目~~

摘　　要：目的:观察逆灸命门穴对力竭游泳大鼠肝糖原、肌糖原及血清ghrelin含量的影响。方法:将大鼠按游泳能力随机分为空白对照组、逆灸命门组、力竭对照组、逆灸命门+力竭组,分别干预20d,第21天进行1次力竭游泳后取材,采用酶联免疫法测定血清ghrelin的含量,采用生化法检测肝脏、比目鱼肌糖原的含量。结果:与空白对照组相比,逆灸命门组大鼠肌糖原、肝糖原和血清ghrelin含量均无明显变化,力竭对照组大鼠肌糖原和肝糖原含量均出现明显下降(P<0.05,P<0.01),血清ghrelin的含量显著升高(P<0.01);与力竭对照组比较,逆灸命门穴+力竭组大鼠肌糖原和肝糖原的含量有所升高(P<0.05),血清中ghrelin的含量显著降低(P<0.01)。结论:逆灸命门穴提高机体运动耐力的机制可能与调用机体不同部位的糖原有关,脑肠肽ghrelin参与糖原调用,推测ghrelin可能是机体力竭状态下能量调控的关键因子。Objective： To observe the effects of preventive moxibustion at Mingmen （DU-4） on liver glycogen, muscle glycogen and serum ghrelin of exhaustive swimming rats. Methods： Randomly divided rats into normal control group, Mingmen moxibustion group, exhaustion control group, Mingmen moxibustion＋exhaustion group according to their swimming exhausted time. Rats received an exhaustive swimming after 20 days of intervention. The serum ghrelin were detected by ELISA method. The liver glycogen and muscle glycogen of rats were detected by biochemical method and compared. Results： Compared with normal control group, the liver glycogen, muscle glycogen and serum ghrelin of Mingmen moxibustion group had no significant changes. The liver glycogen and muscle glycogen of the exhaustion control group were significantly decreased （P〈0.05, P〈0.01）, and the serum ghrelin of the exhaustion control group was significantly increased （P〈0.01）. Compared to the exhaustion control group, the liver glycogen and muscle glycogen of Mingmen moxibustion＋exhaustion group were significantly increased （P〈0.05）, and the serum ghrelin of Mingmen moxibustion＋exhaustion group was significantly decreased （P〈0.01）. Conclusion： The mechanism of moxibustion on Mingmen （DU-4） could improve the exercise tolerance may be associated with the glycogen on different parts of the body. As a brain gut peptide, ghrelin is involved in the metabolism of glycogen. Ghrelin may be the key factor of energy regulation on exhausted state.

关 键 词：力竭游泳 逆灸 命门穴 糖原 GHRELIN 

分 类 号：R245[医药卫生—针灸推拿学]