黑蒜提取液联合放疗抑制荷H22小鼠移植瘤生长的分子机制  

Tumor-inhibiting molecular mechanism research on H22 xenografts with the help of combination of black garlic extract and radiation

作　　者：王义善[1] 杨桂青[1] 王园园[1] 邹嫣丽[1] 杨柯[1] 

机构地区：[1]解放军第107医院国家中西医结合肿瘤治疗重点专科,烟台264002

出　　处：《中华中医药杂志》2013年第11期3235-3239,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：全军科技攻关项目(No.06G034)~~

摘　　要：目的:研究黑蒜提取液联合放疗对荷H22小鼠移植瘤生长的影响,并初步探讨其分子机制。方法:建立荷H22小鼠皮下移植瘤模型,对4组移植瘤模型分别给予0.9%氯化钠溶液、γ射线照射、黑蒜提取液及黑蒜提取液联合γ射线照射的处理,2周后摘取眼球取血后脱臼处死小鼠。剥离瘤体,计算肿瘤生长抑制率;流式细胞术Annexin V-FITC/PI双染法检测肿瘤组织细胞凋亡情况;免疫组织化学法检测肿瘤组织中Bax及Bcl-2蛋白的表达情况;紫外分光光度计法检测各组小鼠血清中SOD及CAT的活力变化。结果:黑蒜提取液联合放疗可显著抑制荷H22小鼠移植瘤的生长,肿瘤生长抑制率高达66.93%;并能够明显诱导H22细胞凋亡,凋亡率可达(53.49±1.98)%,且上调移植瘤组织中Bax及下调Bcl-2蛋白的表达;黑蒜提取液联合放疗可显著提高小鼠血清中SOD和CAT的活力(P<0.05),增强小鼠抗氧化能力。结论:黑蒜提取液联合放疗可明显抑制荷H22小鼠移植瘤的生长,其作用机制可能与其下调抗凋亡Bcl-2蛋白及上调促凋亡Bax蛋白的表达和提高机体抗氧化能力有关。Objective： To explore the effects on tumor growth with H22 by the combination of black garlic extract and radiotherapy and preliminary discuss the possible molecular mechanism. Methods： The tumor-bearing H22 mouse modle was established, 40 mice were randomized into 4 groups： control group （peritoneal injection 0.9%NS, 0.2mL）, irradiation group （ peritoneal injection 0.9%NS, 0.2mL＋yray, 2Gy/day）, black garlic extract group（peritoneal injection, lg/mL black garlic extract 0.2mL）, Black garlic extract ＋ irradiation group （peritoneal injection, lg/mL black garlic extract 0.2mL ＋Tray, 2Gy/day）. After two weeks, mice were sacrificed after eye blood had been gathered, then the tumors were separated and weighed to calculate the tumor-inhibiting rate. Flow cytometry Annexin V-FITC/PI was used to detect tumor cell apoptosis. Immunohistochemistry methods were used in detecting tumor Bax and Bcl-2 protein expression. Ultraviolet spectrophotometer method was used to detect the vigor change of SOD and CAT in mice serum. Results： Black garlic extract combined with radiotherapy could significantly increase the tumor-inhibiting rate, the tumor-inhibiting rate reached as high as 66.93%. The combination of black garlic extract and radiotherapy significantly induced H22 cell apoptosis with the apoptosis rate （53.49＋1.98）%, upregulating the expression of Bax and downregulating the expression of Bcl-2 （P〈0.05）. Black garlic extract combined radiotherapy could enhance the antioxidant ability by improving the mice serum SOD and CAT vigor. Conclusion： Black garlic extract combined radiotherapy can obviously increase the tumor-inhibiting rate of H22 mice. The effect is probably mediated by upregulating the expression of Bax and dowrtregulating the expression of Bcl-2. It also proves to be associated with oxidation resistance.

关 键 词：黑蒜提取液 放射治疗 小鼠H22肝癌细胞 免疫功能 抗氧化 

分 类 号：R285.5[医药卫生—中药学]