甘蔗SCL25基因的克隆与生物信息学分析  

Cloning and Bioimformation Analysis of a Serine/Arginine-Rich Protein Gene SCL25 in Saccharum spp.

作　　者：刘娟 吴嘉云[1,2] 刘少谋[2] 邓祖湖 廖振阳 符成[2] 李虎 黄忠兴[2] 林彦铨 陈如凯 

机构地区：[1]农业部福建甘蔗生物学与遗传育种重点实验室,福州350002 [2]广州甘蔗糖业研究所,广州510316

出　　处：《生物技术通报》2013年第11期51-57,共7页Biotechnology Bulletin

基　　金：国家甘蔗产业技术研发体系专项资金(CARS-20-1-5);国家自然科学基金项目(30671329)

摘　　要：结合电子克隆及RT-PCR获得了甘蔗(Saccharumspp.)花穗中表达的一个SR基因成员。该基因cDNA全长960 bp,包含编码209个氨基酸的开放读码框,gDNA序列长2 966 bp,包含5个内含子。同源性比对表明ScSCL25属于SCL亚家族,命名为ScSCL25(gb:JQ031566)。应用生物信息学工具分析表明:ScSCL25编码蛋白为不稳定亲水性蛋白,含有33个潜在磷酸化位点;无信号肽无跨膜结构域,亚细胞定位于细胞核。该蛋白含有SR基因典型的RRM结构域和SR富集结构域,与玉米等作物的SR蛋白具有较高的同源性。A novel gene of Serine/Arginine-rich proteins, expressed in the inflorescence of Saccharum spp., was cloned. The gene with full-length of 960 bp, encoding 209 aa, might belong to the SCL subfamily of SR protein, inferred by alignment of SR protein members, named ScSCL25 （ gb ： JQ031566 ） . The gDNA sequence is 2 966 bp and contains five introns. Bioinformatics analysis shows that ScSCL25 protein is an unstable hydrophilic protein. There were 33 serine and tyrosine kinase phosphorylation sites. Neither signal peptides nor transmembrane domains been found in this protein sequence, which lead to prediction that it is localized in the cell nucleus. In the space structure, the ScSCL25 contained the typical RRM and RS domain of SR protein, showed high homology to the SR proteins reported in Zea mays.

关 键 词：甘蔗 ScSCL25基因 克隆 生物信息学 

分 类 号：S566.1[农业科学—作物学]