牦牛A-FABP基因的克隆与生物信息学分析  

Cloning and Bioinformatics Analysis of A-FABP Gene in Yak

作　　者：秦文[1,2,3] 阎萍[1,2,3] 裴杰[2,3] 吴晓云[2,3] 李天科[1,2,3] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [3]甘肃省牦牛繁育工程重点实验室,兰州730050

出　　处：《生物技术通报》2013年第11期86-91,共6页Biotechnology Bulletin

基　　金：国家肉牛牦牛产业技术体系牦牛选育(nycytx-38);甘肃牧区生产生态生活优化保障技术集成示范项目(2012BAD13B05)

摘　　要：以牦牛的脂肪型脂肪酸结合蛋白(A-FABP)基因为研究对象,根据GenBank收录的牛的A-FABP基因的mRNA序列设计一对特异性引物,以牦牛背最长肌为材料,采用RT-PCR方法克隆牦牛A-FABP基因的CDS区,并对其进行生物信息学分析。结果表明,牦牛A-FABP基因的CDS区全长399 bp,编码132个氨基酸;其编码的蛋白属于亲水性蛋白。二级结构主要由α-螺旋、β-折叠、延伸链以及无规卷曲组成,含有11个磷酸化位点。牦牛A-FABP基因编码的氨基酸序列与黄牛和水牛的同源性最高且都为97%,与其他物种的同源性也较高,说明在进化关系上A-FABP氨基酸序列较保守,此蛋白序列具Cytosolic fatty-acid binding家族蛋白功能结构域,无信号肽。A-FABP （ Adipocyte Fat Acid Binding Protein ） gene of yak was used to design a pair of specific primer based on the A-FABP mRNA sequences included in NCBI GeneBank. The CDS sequences of yak A-FABP gene was cloned by reverse transcription polymerase chain reaction （ RT-PCR ） from yak longissimus dorsi muscle, and were analyzed. The results showed that, the length of CDS in yak A-FABP mRNA was 399 bp and encoding 132 amino acid, which encoded a hydrophilic protein. The secondary structures were mainly composed of the helix, 13 turn, extended strand and random coil, containing 11 phosphorylation sites. The A-FABP and realated proteins of Bos grunnien, Bos taurus and Bubalus bubalis shared the highest homology of 97%. A-FABP amino acid sequences are preferably conserved on the evolutionary relationships, and also showed high homology of with other species. The A-FABP features cytosolic fatty-acid binding family protein functional domains, no signal peptide.

关 键 词：牦牛 A—FABP基因 克隆 生物信息学分析 

分 类 号：S823.85[农业科学—畜牧学]