辐射固定化大肠杆菌制备菌体膜研究  

THE STUDY OF PREPARATION FOR IMMOBILIZED CELLS MEMBRANES OF E.COLI BY RADIATION TECHNIQUE

作　　者：曹瑾[1] 陈频[1] 俞晔[1] 

机构地区：[1]华东化工学院

出　　处：《辐射研究与辐射工艺学报》1991年第4期219-222,共4页Journal of Radiation Research and Radiation Processing

摘　　要：本文以大肠杆菌作为辐射制备固定化菌体膜的模型,采用尼龙为骨架,接枝甲基丙烯酸β羟乙酯(HEMA),使接枝后的尼龙具有亲水性,而后以低温包埋法固定大肠杆菌成为菌体膜。实验结果表明,当辐射剂量在10—12kGy时,大肠杆菌膜保留活力最高;用50％HEMA单体浓度包埋时,其包埋量达2.3g/glHEMA;菌体膜最适温度与最适pH与原始菌体基本相同。The paper decribed the preparation of immobilized cells membraneswith E. Coli by radiation technique. The nylon 6 was grafted with HEMA, which asmatrix to prepare immobilized cells membranes with E. Coli. by radiation entrapmentat low temperature. The results showed that the retentive activity possessed a maxiumvalue for membranes with E. Coli. when the irradiation dose was at 10-12 kGy, theentrapped cells has 2.3 g/ml at 50% HEMA concentration, the optimum pH andoptimum temperature for membranes with E. Coli. are as same the original cells.

关 键 词：大肠杆菌 辐射固定化 菌体膜 

分 类 号：Q503[生物学—生物化学]