生物样品中氟尿嘧啶的HPLC测定方法建立及鼠体内药物动力学研究  

Establishment of HPLC for the Determination of Fluorouracil in Biological Sample s and Study on Its Pharmacokinetics in Mice

作　　者：余江南[1] 徐希明[1] 朱小霞[2] 吴晓云 张钧寿[3] 

机构地区：[1]镇江医学院药学系,镇江212001 [2]镇江医学院附属医院化疗科,镇江212001 [3]中国药科大学药剂学教研室,南京210009

出　　处：《中国药科大学学报》2000年第6期434-437,共4页Journal of China Pharmaceutical University

基　　金：江苏省青年科技基金资助项目!(项目编号:BQ98043) ;江苏省社会发展基金资助项目!(项目编号:BS98391);镇江医学院博士科研启动基金资

摘　　要：采用离子抑制技术,以5-溴尿嘧啶(5-Bru)为内标,建立了血 浆及组织中5-氟尿嘧啶(5-Fu)的高效液相色谱测定方法。色谱柱: NOVA PAK C18( 3.9　mm×15　cm, 5　μm); 流动相: 0.013 mol/L K2HPO4; 流速: 0.7　ml/min; 检 测波长 : 265　nm。样品经乙酸乙酯提取后, 血浆、心、肝、肺、肾、脾、脑的平均回收率分别为9 5.3％，89.6％，97.4％，92.5％，91.7％，86.1％，79.6％;5-Fu最低检测浓度分别为 2 0，36，28，16，25，30，20 ng/ml。 运用本文建 立的方法进行了5-Fu鼠体内药物动力学研究。An ion suppress high performance liquid chromatographic meth od, using 5-bromouracil(5-Bru) as internal standard, was developed for the determination of fluorouracil(5-Fu) in mice plasma and tissues. Chromatogra phy was performed on a NOVA PAK C18 column (3.9 mm×15 cm i.d., 5 μm) with 0. 013 mol/L K2HPO4 as mobile phase. The flow rate of mobile phase was 0.7 ml/ min. The detection wavelength was 265 nm. The samples were treated with ethyl a cetate. The average recoveries of 5-Fu were 95.3％, 89.6％,97.4％,92.5％,91. 7％,86.1％,79.6％, in plasma, spleen, lung, liver, heart, kidney and brain, an d the detection limit concentrations were 20, 36, 28,16,25,30,20 ng/ml, res pectively. The established method was applied successfully to a pharmacokinetics study of 5-Fu in mice.

关 键 词：氟尿嘧啶 生物样品 药物动力学 HPLC 

分 类 号：R927[医药卫生—药学]