高产类金属硫蛋白假丝酵母菌株的筛选  

Screening of high metallothionein-like protein-producing Candida utilis strains

作　　者：李靖元[1] 张东杰[2] 王颖[2] 郑国君[2] 

机构地区：[1]黑龙江八一农垦大学研究生院农产品加工与贮藏工程,黑龙江大庆163319 [2]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2013年第11期1585-1587,1592,共4页Chinese Journal of Biologicals

摘　　要：目的筛选高产类金属硫蛋白(metallothionein,MT)假丝酵母菌株。方法以产朊假丝酵母菌为出发菌株,利用紫外照射诱变、亚硝基胍(nitrosoguanidine,NTG)化学诱变交替进行的方法进行诱变育种,检测总蛋白含量、巯基活性及类MT含量,筛选高产类MT的菌株。对筛选出的菌株传5代,分析其遗传稳定性。结果获得1株高产类MT的菌株N′′-6,其产类MT的能力由出发菌株的39.6 ng/L提高至165.2 ng/L,巯基活性由出发菌株的0.035μmo/L提高至0.147μmol/L。菌株N′′-6传5代,其总蛋白含量、巯基活性及类MT含量变异度较小。结论筛选出1株高产类MT的假丝酵母菌株,该菌株遗传稳定性较好,为类MT的工业化生产奠定了基础。Objective To screen high metallothionein （MT）-like protein-producing Candida utilis strains. Methods C. utilis as a starting strain was subjected to mutagenesis by ultraviolet irradiation and nitrosoguanidine （NTG） alternatively, and determined for total protein, thiol activity and MT-like protein content, based on which the high MT-like protein-pro- ducing strains were screened, subcuhured for five passages and analyzed for genetic stability. Results A high MT-like protein-producing C. utilis strain N″-6was screened, of which the MT-like protein-producing ability increased from 39. 6 to 165. 2 ng/L, and thiol activity from 0. 035 to 0. 147 μmol/L as compared with those of starting strain. The total pro- tein content, thiol activity and MT-like protein content of strain N″-6 showed no significant variation after subculture for five passages. Conclusion A high MT-like protein-producing C. utilis was screened, which showed high genetic stability and laid a foundation of industrial production of MT-like protein.

关 键 词：假丝酵母菌 金属硫蛋白 紫外线 亚硝基胍 诱变 

分 类 号：Q789[生物学—分子生物学]