在体外受精失败周期中应用卵母细胞质内精子注射的探讨  

Evaluation of the Clinical Use of Intracytoplasmic Sperm Injection after Fertilization Failure in vitro

作　　者：高士友[1] 盛燕[1] 李媛[1] 冯云[1] 陈子江[1] 

机构地区：[1]山东省立医院生殖医学中心,济南250021

出　　处：《生殖与避孕》2000年第5期277-279,305,共4页Reproduction and Contraception

摘　　要：目的　研究卵母细胞质内精子注射（ｉｎｔｒａｃｙｔｏｐｌａｓｍｉｃ　ｓｐｅｒｍ　ｉｎｊｅｃｔｉｏｎ，ＩＣＳＩ）技术用于体外受精失败后的补救措施在临床治疗中的应用。方法　采用卵母细胞质内精子注射（ＩＣＳＩ）技术对体外受精失败的卵子行“再授精”补救，选择外观形态正常的早期胚胎进行宫腔内移植。结果　１４个体外受精失败周期中共有１２７个未受精ＭＩＩ卵采用卵母细胞质内精子注射（ＩＣＳＩ）技术进行“再授精”补救。其中１０５个卵子（８２．７％）注射后正常存活；７１个卵子（５５．９％）正常受精；１５个卵子（１１．８％）为多原核（３原核）受精卵。６１个正常受精卵（８５．９％）发生卵裂。１３个移植周期共移入早期胚胎３２个，获１例临床妊娠（７．７％）。结论　采用卵母细胞质内精子注射（ＩＣＳＩ）技术对体外受精失败后的卵子进行“再授精”作为一种诊断及治疗手段具有一定的临床应用价值，但胚胎移植后的妊娠率较低。Objective To evaluate the clinical use of intracytoplasmic sperm injection when fertilization cycles in vitro failed. Methods Reinsemination by using of intracytoplasmic sperm injection was carried out 24 h after oocytes retrieval in the failed fertilization cycles in vitro. Results A total of 127 one-day-old Mu oocytes from 14 failed fertilization cycles in vitro were subjected to intracyto- plasmic sperm injection. Among them, 105 were survived(82. 7%), 15 polyploidic (11. 8%), and 71 (55. 9%) fertilized normally, of which, 61(85.9%) cleaved. Thirty-two embryos were trans- ferred to 13 patients. One patient (7.7%) conceived after ET. Conclusions Intracytoplasmic sperm injection can be used when fertilization cycles in vitro failed for either diagnostic or thera- peutic purpose, but the pregnancy rates seems not high.

关 键 词：卵母细胞 显微注射 体外受精 妊娠 女性不育 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]