绵羊黄体期卵巢类固醇激素调节基因的表达研究  

Study on Ovarian Sterol-regulatory Genes Expression in Sheep during Luteal Phase

作　　者：应诗家[1] 彭中友[1] 李燕[1] 蔡柳萍[1] 施振旦[1] 

机构地区：[1]江苏省农业科学院畜牧研究所,南京210014

出　　处：《畜牧兽医学报》2013年第11期1775-1780,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑项目(2011BAD19B02-6);江苏省双创人才项目(苏人才办[2011]32号);省六大人才高峰项目(苏农函[2012]35号)

摘　　要：旨在研究黄体期后期湖羊卵巢黄体和不同大小卵泡类固醇激素调节基因表达的变化。选用体重40kg左右的湖羊10头,同期发情结束后第12天屠宰,收集黄体和不同大小卵泡组织。采用Real-time PCR技术检测基因表达水平。试验结果显示,与直径≤2.5mm卵泡相比,直径>2.5mm卵泡的CYP17A1、CYP19A1和VLDLR基因表达水平显著提高(P<0.05),ESR2基因表达水平极显著降低(P<0.01);不同大小卵泡内STAR、CYP11A1、FSHR、LHR、ESR1、LDLR和SR-BI基因表达水平差异不显著(P>0.05),但直径>2.5mm卵泡的ESR1(P=0.090)和SR-BI(P=0.093)基因表达水平高于直径≤2.5mm卵泡;与卵泡相比,黄体几乎不表达CYP17A1和CYP19A1,但STAR、CYP11A1、FSHR、ESR2、LHR、ESR1、LDLR、SR-BI和VLDLR基因在黄体中高表达。试验结果表明类固醇激素调节基因参与调节湖羊黄体期卵泡生长和黄体孕酮合成功能。This study was conducted to investigate the ovine key sterol-regulatroy genes expres- sion from corpora lutea and different-sized follicles in the later luteal phase. 10 cast ages Hu sheep of proven fertility were used. After estrus synchronization, all ewes were slaughtered on day 12 of estrous cycle, and corpora lutea and different-sized follicles were collected at the same time. The gene expression was determined by real-time PCR method. Compared with follicles 2.5 mm in diameter, the follicular very low density lipoprotein receptor （VLDLR）, cytochrome P450 （CYP17A1） and cytochrome P450 aromatase （CYP19A1） mRNA expression were signifi- cantly increased （P^0.05）, however, follicular estrogen receptor 2 （ESR2） mRNA expression was significantly decreased （P~ 0.01）. There were no significant effects of follicle size on follic- ular steroidogenic acute regulatory protein （STAR）, cytochrome P450scc （CYPllA1）, FSH re- ceptor （FSHR）, LH receptor （LHR）, estrogen receptor 1 （ESRI）, low-density lipoprotein re- ceptor （LDLR） and scavenger receptor-BI （SR-BI） mRNA expression, however, ESR1 （P= 0. 090） and SR-BI （P=0. 093） expression trended increase in follicles 〉2.5 mm. CYP19A1 and CYP17A1 were mainly, as expected, expressed in follicles, however, STAR, CYP11A1, FSHR, ESR2, LHR, ESR1, LDLR, SR-BI and VLDLR genes were preferentially expressed in corpora lutea. In conclusion, sterol-regulatory genes took part in mediating follicular growth and progesterone synthesis of corpora lutea.

关 键 词：湖羊 卵巢 类固醇调节基因 

分 类 号：S826[农业科学—畜牧学] S814.1[农业科学—畜牧兽医]