2012年在上海市发现中国大陆首例输入性D8基因型麻疹病例  

Genetic Characterization Analysis on the First Imported Measles Virus of Genotype D8in Chinese Mainland

作　　者：孙晓冬[1] 李崇山[1] 汤显 李智[1] 张燕[3] 唐伟[1] 王静[1] 王慧玲[3] 杨彦基 李加[1] 袁政安[1] 许文波[3] 

机构地区：[1]上海市疾病预防控制中心免疫规划科,上海200336 [2]虹口区疾病预防控制中心免疫规划科,上海200082 [3]中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室世界卫生组织西太平洋地区麻疹风疹地区参比实验室,北京102206

出　　处：《病毒学报》2013年第6期583-588,共6页Chinese Journal of Virology

摘　　要：本文对中国首例输人性D8基因型麻疹病毒基因特征进行分析。用ELISA法检测血清麻疹病毒IgM抗体；用Vero／Slam细胞对采集的咽拭子标本进行病毒分离，分离到的麻疹毒株用RT-PCR方法扩增其核蛋白基因3’端的部分序列，并对扩增产物进行核苷酸序列测定和分析，以3’端456个核苷酸为目的片段进行基因亲缘性关系分析。结果表明，上海市2012年共报告1105疑似麻疹病例，其中实验室确诊590例，临床符合病例2例，排除513例，报告发病率为2．52／10万；共采集到984份疑似麻疹病例咽拭子标本，分离到247株麻疹病毒，病毒分离阳性率为25．3％；除Shanghail2—239为D8基因型外，其他均为Hla基因亚型。Shanghail2—239与世界卫生组织（WorldHealthOrganization，WH0）参考株（Manchester．UNK30．94（D8）AF280803）核苷酸序列同源性为97．8％，氨基酸序列同源性为98．6％。与WHO其他基因型参考株核苷酸序列同源性为89．6％～94．5％，氨基酸序列同源性为88．7％～95．3％。This study analyzed the genetic characterization on first imported measles virus of genotype D8 in Chinese mainland. Serums were collected from the suspicious MV patients to detect IgM antibody in ELISA. Throat swabs were cultured in Vero/SLAM cell line to get measles virus isolates. Part of the nu- cleotide sequence of the 3rterminus of nucleoprotein （N） gene of these isolates were amplified by RT-PCR, and the amplicons were directly sequenced. The phylogenetic analysis was based on the nucleotide sequence about 456 base pairs of the 3rterminus of nucleoprotein （N） gene. Results showed that it reported 1 105 suspicious measles cases in shanghai, 2012, including 590 confirmed cases and 2 clinical case. The reported morbidity was 2.52 per one hundred thousand. 247 measles viruses were isolated from 984 throat swabs specimen. Most of them belonged to sub-genotype Hla except Shanghai12-239 was genotype D8. The ho- mology of nucleotide and amino acid sequences were 97.8% and 98.6% respectively between Shanghai12- 239 and WHO reference strain （Manchester. UNK30.94（D8）AF280803）. Those were 89.6%-94.5% and 88.7%-95.3% between Shanghai12-239 and WHO reference strains of other genotypes.

关 键 词：麻疹病毒 D8基因型 基因特征分析 

分 类 号：R373.9[医药卫生—病原生物学]