本氏烟草KANADI基因的克隆及表达分析  

Cloning and Expression Analysis of KANADI Gene in Nicotiana benthamiana

作　　者：王娟红[1] 丁琦[2] 曾俊[2] 贺新强[2] 李唯[1] 李仲芳[3] 

机构地区：[1]甘肃农业大学生命科学技术学院,兰州730070 [2]北京大学生命科学院,北京100871 [3]天水师范学院,天水741001

出　　处：《农业生物技术学报》2013年第11期1328-1336,共9页Journal of Agricultural Biotechnology

基　　金：国家自然基金项目(No.31070156)

摘　　要：KAN基因参与控制拟南芥侧生器官的发育。为深入研究KAN基因控制植物器官发育的分子机制，本研究采用RT-PCR方法克隆了本氏烟草（Nicotiana benthamiana）KANADI基因家族的全部成员，并对其器官表达谱进行了分析。结果显示，本氏烟草中KANADI基因家族具有8个同源基因，（Genbank登录号分别为：NbS00016721g0007.1、NbS00015047g0006.1、NbS00014907g0003.1、NbS00043258g0009.1、 NbS00006363g0003.1、NbS00042927g0003.1、 NbS00019379g0012.1和NbS00006074g0004.1），每个成员与茄科基因库中对应的同源基因的蛋白序列的相似性均在70%~93%之间，并且具有KANADI家族蛋白特有的保守区和GARP结构域；对该基因家族编码的蛋白结构的生物信息学分析结果显示，该基因家族都编码亲水性的非分泌蛋白，没有明显的跨膜区；实时荧光定量PCR检测显示，该家族基因在本氏烟草各器官中均有表达，但具有不同的表达模式。本研究为进一步深入研究KANADI基因的功能及与其他基因家族之间的相互关系提供了重要依据。KANADI（KAN） genes are involved in controlling the dorsal-ventral polarity of plant lateral organs in Arabidopsis. For further research on the molecular mechanism of plant leaf polarity establishment, all members of KANADI family in Nicotiana benthamiana were cloned by RT-PCR method and their organ expression profile was examined. The Results showed that there were 8 KANADI gene family members in N. benthamiana genome, their encoding proteins shared around 70%~93% similarity to the homologs in Solanaceae and bear the specific conserved region and GARP domain that were found in plant KANADI protein family. Bioinformatic analysis indicated that NbKAN were non-secreted hydrophilic proteins without identifiable transmembrane structures. The results of Real-time qRT-PCR showed that NbKAN genes were present in all plant organs examined and they displayed different expression patterns.Therefore, the experiment provides the important basis for further study the relationships of KANADI gene function and other gene family.

关 键 词：本氏烟草 KANADI基因家族 基因克隆 

分 类 号：S852.659.6[农业科学—基础兽医学]