基质结合区(MAR)序列及其对动物转基因表达的影响  

Review of Matrix Attachment Regions(MAR) Sequence and the Effect of MAR on Transgenic Expression in Transgenic Animals

作　　者：胡晓明[1] 谌颜 高丽[1] 扈廷茂[1] 李光鹏[1] 

机构地区：[1]内蒙古大学生殖生物学及生物技术教育部重点实验室/农业部草食家畜繁殖生物技术与育种重点实验室,呼和浩特010070

出　　处：《农业生物技术学报》2013年第11期1384-1388,共5页Journal of Agricultural Biotechnology

基　　金：高产优质肉牛新品种培育课题(No.2013ZX08007-002)

摘　　要：基质结合区(matrix attachment regions,MAR)是一段能与核基质特异性结合的DNA序列,在进化上较为保守,对于真核生物基因组中的染色体折叠有着非常重要的作用。将其构建到外源基因两侧时可以增加转基因的表达水平,并在一定程度上降低个体之间的差异。目前MAR序列已成为动物和植物基因工程领域的研究热点之一。本文主要综述MAR序列的特征、分布及功能、对转基因动物的影响及作用机制等。MAR （matrix attachment regions） are specific DNA sequences that can combine with nuclear matrix. They are very conservative on the evolution, and they have a very important role in chromosome fold of the eukaryotic genomes. When the MAR sequences are constructed to both sides of the transgene, the expression of transgene will be increased and the differences among individuals can be reduced. Therefore, MAR have become one of the highlights research in the field of animals and plants gene engineering. This review was mainly introduced the following aspects of MAR：the characteristics, the distribution and function, the influence of transgenic animals and the possible mechanisms involved.

关 键 词：基质结合区 基因表达 转基因动物 

分 类 号：S814.8[农业科学—畜牧学]