对硝基苯甲酸与盐酸羟胺对分枝杆菌菌群进行初筛的比较  

Comparison of P-nitrobenzoic acid and hydroxylamine hydrochloride for the primary screening of mycobacterial species

作　　者：吴龙章[1] 谭守勇[1] 谭耀驹[1] 简奕娈[1] 邝璐 潘美玉[1] 曾少芳[1] 刘燕文[1] 钟炳棠[1] 

机构地区：[1]广州市胸科医院检验科,510095 [2]广州市妇女儿童医疗中心

出　　处：《中华结核和呼吸杂志》2013年第11期833-835,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：广东省科技计划项目（2012A030400035）;广州市医药卫生科技项目（20121A011082）

摘　　要：目的 对中国防痨协会制定的分枝杆菌菌群初筛的方法学进行验证和对比研究,为提高实验室初筛分枝杆菌菌群的准确性提供科学依据.方法 以0.5 g/L对硝基苯甲酸（PNB）培养基为对照组,3种浓度的盐酸羟胺培养基（125、150和175 mg/L）为实验组,对广州市胸科医院MTB实验室保存的H37Rv株l株、非结核分枝杆菌标准株12株和临床分离株4295株,同时进行PNB和盐酸羟胺的敏感性试验.组间比较采用x2检验.结果 2529株MTB对PNB培养基的耐受率为3.0％（76/2529）,对125、150和175 mg/L盐酸羟胺培养基的耐受率分别为12.2％（308/2529）、4.8％（121/2529）和0.9％ （23/2529）；1766株非结核分枝杆菌对PNB培养基的敏感率为8.3％ （147/1766）,对125、150和175 mg/L盐酸羟胺培养基的敏感率分别为0.1％（2/1766）、0.5％ （9/1766）和0.9％（16/1766）.3种浓度的盐酸羟胺培养基与PNB培养基比较,差异均有统计学意义（x2值为5.44～83.50,均P＜0.05）.结论 实验室对分枝杆菌菌群进行初筛,宜使用175 mg/L浓度的盐酸羟胺培养基.Objectives To examine the screening methods for mycobacteria recommended by the China Anti-tuberculosis Association,in order to increase laboratory diagnostic accuracy for mycobacterial screening.Methods Using P-nitrobenzoic acid （PNB 0.5 g/L） as the control group,and hydroxylamine hydrochloride （HA,in 125,150 and 175 mg/L concentrations） as the study group,laboratory preserved strains of H37 Rv M.tuberculosis,and standard and clinically isolated strains of M.nontuberculosis （NTM）from Guangzhou Chest Hospital were tested for both PNB and HA sensitivity.Differences between groups were analyzed by x2 test.Results Among the 2529 MTB strains,the resistance rate to PNB was 3.0% （76/2529）,to HA was 12.2% （308/2529）,4.8% （121/2529）,and 0.9% （23/2529）,respectively,corresponding to the aforementioned 3 different concentrations of HA.Among the 1766 NTM strains,the sensitive rate to PNB was 8.3% （147/1766）,to HA was 0.1% （2/1766）,0.5% （9/1766）,and 0.9% （16/1766）,respectively,corresponding to the aforementioned 3 different concentrations of HA.There was significant difference （x2 =5.44-83.50,P 〈 0.05）.Conclusion HA at 175 mg/L concentration was the optimal condition for laboratory tuberculosis preliminary screening.

关 键 词：分枝杆菌属 硝基苯甲酸盐 羟胺 

分 类 号：R446.5[医药卫生—诊断学]