大川芎方沿体外-血浆-脑脊液-脑组织的移行成分研究  

Shift of effective ingredients of Dachuanxiong Decoction along in vitro-plasma-cerebrospinal fluid-brain tissue

作　　者：王强[1] 沈岚[1,2] 房鑫[1] 洪燕龙[2] 冯怡[2] 阮克锋[2] 

机构地区：[1]上海中医药大学中药学院,上海201203 [2]上海中医药大学中药现代制剂技术教育部工程中心,上海201203

出　　处：《中成药》2013年第11期2364-2371,共8页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金青年科学基金项目(30801548);上海市科委科技创新行动计划12401900402;上海市教育委员会科研创新项目(12ZZ124;11zz111);上海市自然基金(11ZR1434500);上海市教委重点学科资助(J50302)

摘　　要：目的研究大川芎方(川芎、天麻)效应组分在硝酸甘油致实验性偏头痛模型大鼠血内、脑内移行成分归属,追溯其从体外-血浆-脑脊液-脑组织的移行趋势。方法以HPLC-DAD为研究手段,建立大川芎方效应组分体内外指纹图谱,并与9个对照品进行成分确认。结果确定大川芎方效应组分中源自于川芎的成分追溯为:体外(20个)-血浆(9个)-脑脊液(6个)-脑组织(5个);确认的入脑成分为阿魏酸、洋川芎内酯I、洋川芎内酯H;源自于天麻的成分追溯为:体外(21个)-血浆(4个)-脑脊液(3个)-脑组织(1个),确认的入脑成分为天麻素、天麻苷元。结论该结果可确立大川芎方的入脑成分,为准确阐明大川芎方治疗偏头痛的药效物质基础提供依据。AIM To study the attribution of characteristic effective ingredients of Dachuanxiong Decoction （ Li- gustici chuangxiong rhizome , Gastrodiae rhizome） （DCXD , and trace back the migratory pathways from in vitro- plasma-cerebrospinal fluid-brain tissue in experimental rats migraine model induced by nitroglycerin. METHODS The HPLC-DAD was used as the analytic methed to establish in vitro and in vivo fingerprint chromatograms of DCXD, and nine constituents were confirmed. RESULTS The in vitro （20 constituents） -plasma （9） -cerebro- spinal fluid （6） -brain tissue （5） could date back to Ligustici chuangxiong rhizome and had been verified that the ingredients absorbed into brain were ferulic acid, senkyunolide I and senkyunolide H （C15）. Meanwhile in vitro （21 constituents） -plasma （4） -cerebrospinal fluid （3） -brain tissue （1） could date back to Gastradine rhizome. And it was confirmed that the ingredients brought into the brain were gastrodin and 4-hydroxyphenethyl alcohol. CONCLUSION The findings could provide the useful information for the determination of bioactive substances of DCXD which is used to treating migraine.

关 键 词：大川芎方 移行成分 归属 指纹图谱 

分 类 号：R969.1[医药卫生—药理学]