射干有效组分大孔吸附树脂分离特性的研究  

Study on Separation and Purification of Effective Substance from Belamcanda chinensis by Macroporous Absorption Resin

作　　者：尤献民[1] 邹桂欣[1] 姜鸿[1] 张颖[1] 李国信[1] 

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《辽宁中医杂志》2013年第11期2328-2330,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重大新药创制-科技重大专项(2009ZX9103-330);辽宁省科技厅社会发展攻关计划(2009226051)

摘　　要：目的:研究大孔吸附树脂富集纯化射干抗炎、抗病毒、止咳及抑菌总黄酮药效物质成分的工艺参数。方法:分别以射干中抗炎、抗病毒、止咳及抑菌药效物质成分组为指标,采用动态吸附及静态吸附实验,对工艺条件进行考查。结果:7种比例的D101大孔吸附树脂和聚酰胺中,D101大孔吸附树脂具有最佳的吸附及洗脱参数,最佳吸附条件:药液浓度为0.4 g·mL-1;最佳洗脱条件:用6 BV的20%乙醇洗涤除质,再用8 BV的80%乙醇以5 BV·h-1洗脱。结论:本方法可得到较纯的射干抗炎、抗病毒、抑菌药效物质有效部位群,方法简单易行。Objective ： To investigate the technological parameters of the purification process of effective substance from Belam- canda chinensis with macroporous absorption resin. Methods ：The technical process for purification of effective substance was with the optimal macroporotls absorption. Results ：The D101 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0.4 g · mL^- 1 （equivalent to raw material） , and the volume of 80% ethanol as eluant 8 BV with desorption -power 5 BV· h^-1. Conclusion：The treatment o f regenerated resin is easy and this method is advisable.

关 键 词：大孔树脂分离 药效物质成分 射干 

分 类 号：R284[医药卫生—中药学]